BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25393

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for IST1 residues 303-366   PubMed: 26011858

Deposition date: 2014-12-15 Original release date: 2015-05-26

Authors: Wenzel, Dawn; Skalicky, Jack; Sundquist, Wesley

Citation: Caballe, Anna; Wenzel, Dawn; Agromayor, Monica; Alam, Steven; Skalicky, Jack; Kloc, Magdalena; Carlton, Jeremy; Labrador, Leticia; Sundquist, Wesley; Martin-Serrano, Juan. "ULK3 regulates cytokinetic abscission by phosphorylating ESCRT-III proteins"  Elife 4, e06547-e06547 (2015).

Assembly members:
IST1, polymer, 67 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
IST1: GHMGPKPEASAKLPSRPADN YDNFVLPELPSVPDTLPTAS AGASTSASEDIDFDDLSRRF EELKKKT

Data sets:
Data typeCount
13C chemical shifts184
15N chemical shifts56
1H chemical shifts56

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1IST1 peptide1

Entities:

Entity 1, IST1 peptide 67 residues - Formula weight is not available

1   GLYHISMETGLYPROLYSPROGLUALASER
2   ALALYSLEUPROSERARGPROALAASPASN
3   TYRASPASNPHEVALLEUPROGLULEUPRO
4   SERVALPROASPTHRLEUPROTHRALASER
5   ALAGLYALASERTHRSERALASERGLUASP
6   ILEASPPHEASPASPLEUSERARGARGPHE
7   GLUGLULEULYSLYSLYSTHR

Samples:

sample_1: IST1, [U-13C; U-15N], 0.4 mM; sodium phosphate 20 mM; NaCl 25 mM; EDTA 0.1 mM; DTT 0.5 mM; sodium azide 0.1 mM

sample_conditions_1: ionic strength: 0.025 M; pH: 6.2; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts