BMRB

Biological Magnetic Resonance Data Bank


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BMRB Entry 25432

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25432

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Title: Solution NMR assignment of the 6th TOG domain of minispindles   PubMed: 25971232

Deposition date: 2015-01-16 Original release date: 2016-06-30

Authors: Burgess, Selena; Bayliss, Richard; Pfuhl, Mark

Citation: Burgess, Selena; Bayliss, Richard; Pfuhl, Mark. "Solution NMR assignment of the cryptic sixth TOG domain of mini spindles"  Biomol. NMR Assign. 9, 411-413 (2015).

Assembly members:
TOG6, polymer, 261 residues, Formula weight is not available

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TOG6: ADVLPKHDPQLVKVIKGVSS TDTLKARAAINELAAIIEAP EKQAVLRDYEEIFIQNVLAQ FKNLSQIPSAQSVVVYQPLL SILYTFFHANILGKTLSVAC IKNLMSALLNLMADPKLAVG DDSQYNKVINGICLKVLDKV DFTNLNCALIRLLRETCPEA KLPKFTDLLMKCIWRNVKML PERSNELNYDAVILEVHEFM LALPSTWWQNRPSDTPMRTI KTILHNMAKVKGNAILQHLN QIPTHSELHTYLIRILKNFQ K

Data sets:
Data typeCount
13C chemical shifts1120
15N chemical shifts253
1H chemical shifts1879

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 261 residues - Formula weight is not available

1   ALAASPVALLEUPROLYSHISASPPROGLN
2   LEUVALLYSVALILELYSGLYVALSERSER
3   THRASPTHRLEULYSALAARGALAALAILE
4   ASNGLULEUALAALAILEILEGLUALAPRO
5   GLULYSGLNALAVALLEUARGASPTYRGLU
6   GLUILEPHEILEGLNASNVALLEUALAGLN
7   PHELYSASNLEUSERGLNILEPROSERALA
8   GLNSERVALVALVALTYRGLNPROLEULEU
9   SERILELEUTYRTHRPHEPHEHISALAASN
10   ILELEUGLYLYSTHRLEUSERVALALACYS
11   ILELYSASNLEUMETSERALALEULEUASN
12   LEUMETALAASPPROLYSLEUALAVALGLY
13   ASPASPSERGLNTYRASNLYSVALILEASN
14   GLYILECYSLEULYSVALLEUASPLYSVAL
15   ASPPHETHRASNLEUASNCYSALALEUILE
16   ARGLEULEUARGGLUTHRCYSPROGLUALA
17   LYSLEUPROLYSPHETHRASPLEULEUMET
18   LYSCYSILETRPARGASNVALLYSMETLEU
19   PROGLUARGSERASNGLULEUASNTYRASP
20   ALAVALILELEUGLUVALHISGLUPHEMET
21   LEUALALEUPROSERTHRTRPTRPGLNASN
22   ARGPROSERASPTHRPROMETARGTHRILE
23   LYSTHRILELEUHISASNMETALALYSVAL
24   LYSGLYASNALAILELEUGLNHISLEUASN
25   GLNILEPROTHRHISSERGLULEUHISTHR
26   TYRLEUILEARGILELEULYSASNPHEGLN
27   LYS

Samples:

sample_1: TOG6, [U-98% 15N], 250 ± 15 uM; DTT 2 ± 0.1 mM; sodium chloride 50 ± 1 mM; sodium azide 0.02 ± 0.001 %; H2O 95%; D2O 5%

sample_2: TOG6, [U-95% 13C; U-95% 15N], 250 ± 15 uM; DTT 2 ± 0.1 mM; sodium chloride 50 ± 1 mM; sodium azide 0.02 ± 0.001 %; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.1 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1

Software:

TOPSPIN v3.1, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Varian INOVA 800 MHz
  • Varian INOVA 900 MHz

Related Database Links:

UNP Q9U5W6

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts