BMRB Entry 25445
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25445
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: HN,N,CA,CB assignments of A2A-ct PubMed: 25952762
Deposition date: 2015-01-20 Original release date: 2016-06-30
Authors: Tossavainen, Helena; Hellman, Maarit; Permi, Perttu
Citation: Tossavainen, Helena; Hellman, Maarit; Piirainen, Henni; Jaakola, Veli-Pekka; Permi, Perttu. "HN, N, CA and CB assignments of the intrinsically disordered C-terminus of human adenosine A2A receptor" Biomol. NMR Assign. 9, 403-406 (2015).
Assembly members:
A2A-ct, polymer, 134 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
A2A-ct: MREFRQTFRKIIRSHVLRQQ
EPFKAAGTSARVLAAHGSDG
EQVSLRLNGHPPGVWANGSA
PHPERRPNGYALGLVSGGSA
QESQGNTGLPDVELLSHELK
GVCPEPPGLDDPLAQDGAGV
SGLEHHHHHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 344 |
| 15N chemical shifts | 122 |
| 1H chemical shifts | 111 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | A2A-ct | 1 |
Entities:
Entity 1, A2A-ct 134 residues - Formula weight is not available
| 1 | MET | ARG | GLU | PHE | ARG | GLN | THR | PHE | ARG | LYS | ||||
| 2 | ILE | ILE | ARG | SER | HIS | VAL | LEU | ARG | GLN | GLN | ||||
| 3 | GLU | PRO | PHE | LYS | ALA | ALA | GLY | THR | SER | ALA | ||||
| 4 | ARG | VAL | LEU | ALA | ALA | HIS | GLY | SER | ASP | GLY | ||||
| 5 | GLU | GLN | VAL | SER | LEU | ARG | LEU | ASN | GLY | HIS | ||||
| 6 | PRO | PRO | GLY | VAL | TRP | ALA | ASN | GLY | SER | ALA | ||||
| 7 | PRO | HIS | PRO | GLU | ARG | ARG | PRO | ASN | GLY | TYR | ||||
| 8 | ALA | LEU | GLY | LEU | VAL | SER | GLY | GLY | SER | ALA | ||||
| 9 | GLN | GLU | SER | GLN | GLY | ASN | THR | GLY | LEU | PRO | ||||
| 10 | ASP | VAL | GLU | LEU | LEU | SER | HIS | GLU | LEU | LYS | ||||
| 11 | GLY | VAL | CYS | PRO | GLU | PRO | PRO | GLY | LEU | ASP | ||||
| 12 | ASP | PRO | LEU | ALA | GLN | ASP | GLY | ALA | GLY | VAL | ||||
| 13 | SER | GLY | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 14 | HIS | HIS | HIS | HIS |
Samples:
sample_1: A2A-ct, [U-13C; U-15N], 0.6 mM; HEPES 50 mM; TCEP-HCl 1 mM; sodium chloride 250 mM; calcium chloride 5 mM; H2O 95%; D2O, U-2H, 5%
sample_conditions_1: ionic strength: 300 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D i(HCA)CO(CA)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D (HCA)N(CA)CO(N)H | sample_1 | isotropic | sample_conditions_1 |
| 3D (HCA)CON(CAN)H | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Agilent - processing
SPARKY, Goddard - data analysis
NMR spectrometers:
- Agilent INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts