BMRB Entry 25457
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25457
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Cullin3 - BTB interface: a novel target for stapled peptides PubMed: 25848797
Deposition date: 2015-01-27 Original release date: 2015-04-20
Authors: Russo, Luigi; Palmieri, Maddalena; Malgieri, Gaetano
Citation: de Paola, Ivan; Pirone, Luciano; Palmieri, Maddalena; Balasco, Nicole; Esposito, Luciana; Russo, Luigi; Mazz, Daniela; Di Marcotullio, Lucia; Di Gaetano, Sonia; Malgieri, Gaetano; Vitagliano, Luigi; Pedone, Emilia; Zaccaro, Laura. "Cullin3 - BTB Interface: A Novel Target for Stapled Peptides" PLoS ONE 10, e0121149-e0121149 (2015).
Assembly members:
entity, polymer, 20 residues, 2445.832 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: NSGLSFEEXYRNXYTMVLHK
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 129 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | entity | 1 |
Entities:
Entity 1, entity 20 residues - 2445.832 Da.
1 | ASN | SER | GLY | LEU | SER | PHE | GLU | GLU | MK8 | TYR | |
2 | ARG | ASN | MK8 | TYR | THR | MET | VAL | LEU | HIS | LYS |
Samples:
sample_1: Cul349-68LA 1 mM; trifluoroethanol%; water%
sample_conditions_1: pH: 3; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, refinement, structure solution
NMR spectrometers:
- Varian INOVA 500 MHz
Related Database Links:
PDB |