BMRB Entry 25479
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25479
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Title: Role of a non-canonical surface of Rad6 in ubiquitin conjugating activity PubMed: 26286193
Deposition date: 2015-02-09 Original release date: 2016-06-30
Authors: Kumar, Pankaj; Magala, Pearl; Gieger, Kathryn; Majumdar, Ananya; Tolman, Joel; Wolberger, Cynthia
Citation: Kumar, Pankaj; Magala, Pearl; Gieger, Kathryn; Majumdar, Ananya; Tolman, Joel; Wolberger, Cynthia. "Role of a non-canonical surface of Rad6 in ubiquitin conjugating activity" Nucleic Acids Res. 43, 9039-9050 (2015).
Assembly members:
Rad6, polymer, 169 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Rad6: GSMSTPARRRLMRDFKRMKE
DAPPGVSASPLPDNVMVWNA
MIIGPADTPYEDGTFRLLLE
FDEEYPNKPPHVKFLSEMFH
PNVYANGEICLDILQNRWTP
TYDVASILTSIQSLFNDPNP
ASPANVEAATLFKDHKSQYV
KRVKETVEKSWEDDMDDMDD
DDDDDDDDD
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 76 |
| 1H chemical shifts | 76 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rad6 | 1 |
Entities:
Entity 1, Rad6 169 residues - Formula weight is not available
| 1 | GLY | SER | MET | SER | THR | PRO | ALA | ARG | ARG | ARG | ||||
| 2 | LEU | MET | ARG | ASP | PHE | LYS | ARG | MET | LYS | GLU | ||||
| 3 | ASP | ALA | PRO | PRO | GLY | VAL | SER | ALA | SER | PRO | ||||
| 4 | LEU | PRO | ASP | ASN | VAL | MET | VAL | TRP | ASN | ALA | ||||
| 5 | MET | ILE | ILE | GLY | PRO | ALA | ASP | THR | PRO | TYR | ||||
| 6 | GLU | ASP | GLY | THR | PHE | ARG | LEU | LEU | LEU | GLU | ||||
| 7 | PHE | ASP | GLU | GLU | TYR | PRO | ASN | LYS | PRO | PRO | ||||
| 8 | HIS | VAL | LYS | PHE | LEU | SER | GLU | MET | PHE | HIS | ||||
| 9 | PRO | ASN | VAL | TYR | ALA | ASN | GLY | GLU | ILE | CYS | ||||
| 10 | LEU | ASP | ILE | LEU | GLN | ASN | ARG | TRP | THR | PRO | ||||
| 11 | THR | TYR | ASP | VAL | ALA | SER | ILE | LEU | THR | SER | ||||
| 12 | ILE | GLN | SER | LEU | PHE | ASN | ASP | PRO | ASN | PRO | ||||
| 13 | ALA | SER | PRO | ALA | ASN | VAL | GLU | ALA | ALA | THR | ||||
| 14 | LEU | PHE | LYS | ASP | HIS | LYS | SER | GLN | TYR | VAL | ||||
| 15 | LYS | ARG | VAL | LYS | GLU | THR | VAL | GLU | LYS | SER | ||||
| 16 | TRP | GLU | ASP | ASP | MET | ASP | ASP | MET | ASP | ASP | ||||
| 17 | ASP | ASP | ASP | ASP | ASP | ASP | ASP | ASP | ASP |
Samples:
sample_1: Rad6, [U-100% 15N], 0.15 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.0; temperature: 293.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Thomas Goddard - data analysis
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker AMX 600 MHz
- Varian INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts