BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25569

Title: PIN1 WW domain in complex with a phosphorylated CPEB1 derived peptide   PubMed: 26456073

Deposition date: 2015-04-13 Original release date: 2015-10-26

Authors: Schelhorn, Constanze; Macias, Maria J.; Martin-Malpartida, Pau

Citation: Schelhorn, Constanze; Martin-Malpartida, Pau; Sunol, David; Macias, Maria J.. "Structural Analysis of the Pin1-CPEB1 interaction and its potential role in CPEB1 degradation"  Sci. Rep. 5, 14990-14990 (2015).

Assembly members:
Pin1, polymer, 33 residues, 3901.367 Da.
CPEB1, polymer, 8 residues, 1007.111 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Pin1: LPPGWEKRMSRSSGRVYYFN HITNASQWERPSG
CPEB1: RIXPPLPF

Data sets:
Data typeCount
1H chemical shifts235

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pin11
2CPEB12

Entities:

Entity 1, Pin1 33 residues - 3901.367 Da.

1   LEUPROPROGLYTRPGLULYSARGMETSER
2   ARGSERSERGLYARGVALTYRTYRPHEASN
3   HISILETHRASNALASERGLNTRPGLUARG
4   PROSERGLY

Entity 2, CPEB1 8 residues - 1007.111 Da.

1   ARGILESEPPROPROLEUPROPHE

Samples:

sample_1: Pin1 1 mM; CPEB1 3 mM; D2O, [U-100% 2H], 10%; TRIS, [U-100% 2H], 20 mM; sodium chloride 130 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 285 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

XEASY, Bartels et al. - chemical shift assignment

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

UNP Q13526 Q91572
BMRB 16070 16088 17545 19258 19259
PDB
DBJ BAA87037 BAA87038 BAB22270 BAB22743 BAC35631
EMBL CAG28582
GB AAC50492 AAH02899 AAH38254 AAH59553 AAI12584
PRF 2209428A
REF NP_001029804 NP_001100171 NP_001231300 NP_001270625 NP_006212
SP Q13526 Q4R383 Q5BIN5 Q9QUR7
TPG DAA28013