BMRB Entry 25587
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25587
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Title: Solution structures of Miz-1 zinc fingers 2 to 4
Deposition date: 2015-04-28 Original release date: 2016-03-22
Authors: Bedard, Mikael; Lavigne, Pierre
Citation: Bedard, Mikael; Lavigne, Pierre. "Solution structures of Miz-1 zinc fingers 2 to 4" J. Biol. Chem. ., .-..
Assembly members:
Miz1-4, polymer, 112 residues, 12790.7447 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Miz1-4: MVIHKCEDCGKEFTHTGNFK
RHIRIHTGEKPFSCRECSKA
FSDPAACKAHEKTHSPLKPY
GCEECGKSYRLISLLNLHKK
RHSGEARYRCEDCGKLFTTS
GNLKRHQLVHSG
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 395 |
| 15N chemical shifts | 99 |
| 1H chemical shifts | 524 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Miz1-4 | 1 |
Entities:
Entity 1, Miz1-4 112 residues - 12790.7447 Da.
| 1 | MET | VAL | ILE | HIS | LYS | CYS | GLU | ASP | CYS | GLY | ||||
| 2 | LYS | GLU | PHE | THR | HIS | THR | GLY | ASN | PHE | LYS | ||||
| 3 | ARG | HIS | ILE | ARG | ILE | HIS | THR | GLY | GLU | LYS | ||||
| 4 | PRO | PHE | SER | CYS | ARG | GLU | CYS | SER | LYS | ALA | ||||
| 5 | PHE | SER | ASP | PRO | ALA | ALA | CYS | LYS | ALA | HIS | ||||
| 6 | GLU | LYS | THR | HIS | SER | PRO | LEU | LYS | PRO | TYR | ||||
| 7 | GLY | CYS | GLU | GLU | CYS | GLY | LYS | SER | TYR | ARG | ||||
| 8 | LEU | ILE | SER | LEU | LEU | ASN | LEU | HIS | LYS | LYS | ||||
| 9 | ARG | HIS | SER | GLY | GLU | ALA | ARG | TYR | ARG | CYS | ||||
| 10 | GLU | ASP | CYS | GLY | LYS | LEU | PHE | THR | THR | SER | ||||
| 11 | GLY | ASN | LEU | LYS | ARG | HIS | GLN | LEU | VAL | HIS | ||||
| 12 | SER | GLY |
Samples:
sample_1: Miz1-4, [U-13C; U-15N], 0.7 – 1.0 mM; acetic acid 10 mM; cacodylate 10 mM; KCl 50 mM; TCEP 2 mM; ZnCl2 5 equivalents; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| cbcaconh (H[N[co[{CA|ca[C]}]]]) | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| hnha (H{[N]+[HA]}) | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC/HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| ccconh (HNCO) | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC/HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS v1.21, Brunger, Adams, Clore, Gros, Nilges and Read - Structure calculation, refinement
CcpNmr_Analysis v2.1, CCPN - Chemical shift assigment, data analysis
DANGLE v1.1, CCPN - Data analysis
NMRPipe v7.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Processing
ARIA v2.2, Nilges, Rieping, Habeck, Bardiaux, Bernard and Malliavin - Structure calculation, refinement
NMR spectrometers:
- Varian UnityInova 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts