BMRB Entry 25684
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25684
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Title: SOLUTION STRUCTURE OF THE LIPID DROPLET ANCHORING PEPTIDE OF CGI-58 BOUND TO DPC MICELLES PubMed: 26350461
Deposition date: 2015-07-01 Original release date: 2015-09-14
Authors: Boeszoermenyi, Andras; Arthanari, Haribabu; Wagner, Gerhard; Nagy, Harald; Zangger, Klaus; Lindermuth, Hanna; Oberer, Monika
Citation: Boeszoermenyi, Andras; Arthanari, Haribabu; Wagner, Gerhard; Nagy, Harald; Zangger, Klaus; Lindermuth, Hanna; Oberer, Monika. "Structure of a CGI-58 Motif Provides the Molecular Basis of Lipid Droplet Anchoring" J. Biol. Chem. 290, 26361-26372 (2015).
Assembly members:
wr10_43, polymer, 39 residues, 3976.3072 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI
Entity Sequences (FASTA):
wr10_43: GAMGSVDSADAGGGSGWLTG
WLPTWCPTSTSHLKEAEEK
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 216 |
| 15N chemical shifts | 71 |
| 1H chemical shifts | 662 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | wr10 43 | 1 |
Entities:
Entity 1, wr10 43 39 residues - 3976.3072 Da.
| 1 | GLY | ALA | MET | GLY | SER | VAL | ASP | SER | ALA | ASP | ||||
| 2 | ALA | GLY | GLY | GLY | SER | GLY | TRP | LEU | THR | GLY | ||||
| 3 | TRP | LEU | PRO | THR | TRP | CYS | PRO | THR | SER | THR | ||||
| 4 | SER | HIS | LEU | LYS | GLU | ALA | GLU | GLU | LYS |
Samples:
sample_new_1: wr10_43, [U-13C; U-15N], 1 mM; NaPP 20 mM; NaCl 50 mM; EDTA 1 mM; DTT 5 mM
wr10_43_1: wr10_43, [U-13C; U-15N], 1 mM; NaPP 20 mM; NaCl 50 mM; EDTA 1 mM; DTT 5 mM
wr10_43_D2O: wr10_43, [U-13C; U-15N], 1 mM; NaPP 20 mM; NaCl 50 mM; EDTA 1 mM; DTT 5 mM
wr18_39: wr10_43, [U-13C; U-15N], 1 mM; NaPP 20 mM; NaCl 50 mM; EDTA 1 mM; DTT 5 mM
1: ionic strength: 70.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 310.000 K
2: ionic strength: 70.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 303.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC/HMQC | wr10_43_1 | isotropic | 1 |
| HNCACB (H[N[ca[CB]]]) | wr10_43_1 | isotropic | 1 |
| 3D HNCA | wr10_43_1 | isotropic | 1 |
| 3D 1H-15N NOESY | wr10_43_1 | isotropic | 1 |
| HNCACO (H[N[ca[CO]]]) | wr10_43_1 | isotropic | 1 |
| HCCONH (HccoNH) | wr10_43_1 | isotropic | 1 |
| CCONH (hC_cacoNH.relayed) | wr10_43_1 | isotropic | 1 |
| 3D HCCH-TOCSY | wr10_43_1 | isotropic | 1 |
| 2D 1H-13C HSQC/HMQC | wr10_43_D2O | isotropic | 1 |
| 3D 1H-13C NOESY | wr10_43_D2O | isotropic | 1 |
| 2D 1H-1H NOESY | wr18_39 | isotropic | 2 |
| 2D 1H-15N HSQC/HMQC | wr10_43_1 | isotropic | 1 |
| 2D 1H-1H TOCSY | wr10_43_1 | isotropic | 2 |
| HNCA | sample_new_1 | solution | 1 |
| HNCACB | sample_new_1 | solution | 1 |
| HNCACO | sample_new_1 | solution | 1 |
| HSQC | sample_new_1 | solution | 1 |
Software:
CcpNmr_Analysis v2.4, CCPN - Spectrum analysis, peak integration, distance restraint calibration
Cyana v3.0, GUNTERT - Simulated annealing
TALOS+ v1, Shen, Cornilescu, Delaglio. and Bax - Prediction of dihedral angle restraints
nmrDraw vany, F. Delaglio - Spectrum display
nmrPipe vany, F. Delaglio - Spectrum processing
NMR spectrometers:
- Bruker Avance 600 MHz
- Varian dd2 700 MHz
- Bruker Avance 750 MHz
- Varian UnityInova 500 MHz
- Bruker Avance 900 MHz
- Bruker DD2 600 MHz