BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25722

Title: Unveiling the structural determinants of KIAA0323 binding preference for NEDD8

Deposition date: 2015-07-21 Original release date: 2016-07-25

Authors: Santonico, Elena; Nepravishta, Ridvan; Mattioni, Anna; Valentini, Eleonora; Mandaliti, Walter; Procopio, Radha; Iannuccelli, Marta; Castagnoli, Luisa; Polo, Simona; Paci, Maurizio; Cesareni, Gianni

Citation: Santonico, Elena; Nepravishta, Ridvan; Mattioni, Anna; Valentini, Eleonora; Mandaliti, Walter; Procopio, Radha; Iannuccelli, Marta; Castagnoli, Luisa; Polo, Simona; Paci, Maurizio; Cesareni, Gianni. "Unveiling the structural determinants of KIAA0323 binding preference for NEDD8."  Not known ., .-..

Assembly members:
KIAA0323, polymer, 52 residues, 6299.246 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
KIAA0323: GGIRKTRETERLRRQLLEVF WGQDHKVDFILQREPYCRDI NQLSEALLSLNF

Data sets:
Data typeCount
15N chemical shifts50
1H chemical shifts181

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KIAA03231

Entities:

Entity 1, KIAA0323 52 residues - 6299.246 Da.

The sequence is the C-terminal end of KIAA0323

1   GLYGLYILEARGLYSTHRARGGLUTHRGLU
2   ARGLEUARGARGGLNLEULEUGLUVALPHE
3   TRPGLYGLNASPHISLYSVALASPPHEILE
4   LEUGLNARGGLUPROTYRCYSARGASPILE
5   ASNGLNLEUSERGLUALALEULEUSERLEU
6   ASNPHE

Samples:

sample_1: KIAA0323, [U-100% 15N], 0.16 – 1.2 mM; H2O 90%; D2O 10%; Phosphate Buffer 12 mM

sample_conditions_1: ionic strength: 0.012 M; pH: 5.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - geometry optimization, structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts