BMRB Entry 25771
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25771
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Title: 1H assignment at 288K of Cementoin PubMed: 26878852
Deposition date: 2015-08-26 Original release date: 2016-03-03
Authors: Loth, Karine; Abou Ibrahim Alami, Soha; Habes, Chahrazed; Zani, Marie-Louise; Delmas, Agnes; Moreau, Thierry; Landon, Celine
Citation: Loth, Karine; Abou Ibrahim Alami, Soha; Habes, Chahrazed; Zani, Marie-Louise; Delmas, Agnes; Moreau, Thierry; Landon, Celine. "Complete assignment of 15N, 13C Trappin-2 and 1H assignment of its two domains, Elafin and Cementoin." Biomol. NMR Assign. 10, 223-226 (2016).
Assembly members:
Cementoin, polymer, 38 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Cementoin: AVTGVPVKGQDTVKGRVPFN
GQDPVKGQVSVKGQDKVK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 266 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Cementoin | 1 |
Entities:
Entity 1, Cementoin 38 residues - Formula weight is not available
| 1 | ALA | VAL | THR | GLY | VAL | PRO | VAL | LYS | GLY | GLN | ||||
| 2 | ASP | THR | VAL | LYS | GLY | ARG | VAL | PRO | PHE | ASN | ||||
| 3 | GLY | GLN | ASP | PRO | VAL | LYS | GLY | GLN | VAL | SER | ||||
| 4 | VAL | LYS | GLY | GLN | ASP | LYS | VAL | LYS |
Samples:
sample_1: Cementoin 1.2 mM; sodium chloride 300 mM; sodium phosphate 25 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 300 mM; pH: 6.1; pressure: 1 atm; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
Ccpnmr, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz