BMRB Entry 25773
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25773
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Title: 1H, 13C, 15N assignments of Trappin-2 PubMed: 26878852
Deposition date: 2015-08-26 Original release date: 2016-03-03
Authors: LOTH, Karine; Abou Ibrahim Alami, Soha; Habes, Chahrazed; Zani, Marie-Louise; Delmas, Agnes; Moreau, Thierry; Landon, Celine
Citation: Loth, Karine; Abou Ibrahim Alami, Soha; Habes, Chahrazed; Zani, Marie-Louise; Delmas, Agnes; Moreau, Thierry; Landon, Celine. "Complete assignment of 15N, 13C Trappin-2 and 1H assignment of its two domains, Elafin and Cementoin." Biomol. NMR Assign. 10, 223-226 (2016).
Assembly members:
Trappin-2, polymer, 95 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Pichia pastoris
Entity Sequences (FASTA):
Trappin-2: AVTGVPVKGQDTVKGRVPFN
GQDPVKGQVSVKGQDKVKAQ
EPVKGPVSTKPGSCPIILIR
CAMLNPPNRCLKDTDCPGIK
KCCEGSCGMACFVPQ
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 401 |
15N chemical shifts | 95 |
1H chemical shifts | 652 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Trappin-2 | 1 |
Entities:
Entity 1, Trappin-2 95 residues - Formula weight is not available
1 | ALA | VAL | THR | GLY | VAL | PRO | VAL | LYS | GLY | GLN | ||||
2 | ASP | THR | VAL | LYS | GLY | ARG | VAL | PRO | PHE | ASN | ||||
3 | GLY | GLN | ASP | PRO | VAL | LYS | GLY | GLN | VAL | SER | ||||
4 | VAL | LYS | GLY | GLN | ASP | LYS | VAL | LYS | ALA | GLN | ||||
5 | GLU | PRO | VAL | LYS | GLY | PRO | VAL | SER | THR | LYS | ||||
6 | PRO | GLY | SER | CYS | PRO | ILE | ILE | LEU | ILE | ARG | ||||
7 | CYS | ALA | MET | LEU | ASN | PRO | PRO | ASN | ARG | CYS | ||||
8 | LEU | LYS | ASP | THR | ASP | CYS | PRO | GLY | ILE | LYS | ||||
9 | LYS | CYS | CYS | GLU | GLY | SER | CYS | GLY | MET | ALA | ||||
10 | CYS | PHE | VAL | PRO | GLN |
Samples:
sample_1: Trappin-2, [U-100% 13C; U-100% 15N], 0.3 mM; sodium phosphate 25 mM; sodium chloride 300 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 300 mM; pH: 6.1; pressure: 1 atm; temperature: 288 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNHAHB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
Ccpnmr, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts