BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25816

Title: Solution structure of PDZ domain   PubMed: 26917351

Deposition date: 2015-10-05 Original release date: 2015-10-05

Authors: Mei, Song

Citation: Mei, Song; Dong, Yuanqiu; Zhang, Jiahai; Zhang, Xuecheng; Tu, Xiaoming. "Solution structure of Q388A3 PDZ domain from Trypanosoma brucei"  J. Struct. Biol. 194, 214-217 (2016).

Assembly members:
entity, polymer, 105 residues, 10985.538 Da.

Natural source:   Common Name: Trypanosoma   Taxonomy ID: 5690   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: PLTRPYLGFRVAVGRDSSGC TTLSIQEVTQTYTGSNGGAD LMGPAFAAGLRVGDQLVRFA GYTVTELAAFNTVVARHVRP SASIPVVFSRDGVVMSATIV VGELE

Data sets:
Data typeCount
13C chemical shifts276
15N chemical shifts102
1H chemical shifts572

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 105 residues - 10985.538 Da.

1   PROLEUTHRARGPROTYRLEUGLYPHEARG
2   VALALAVALGLYARGASPSERSERGLYCYS
3   THRTHRLEUSERILEGLNGLUVALTHRGLN
4   THRTYRTHRGLYSERASNGLYGLYALAASP
5   LEUMETGLYPROALAPHEALAALAGLYLEU
6   ARGVALGLYASPGLNLEUVALARGPHEALA
7   GLYTYRTHRVALTHRGLULEUALAALAPHE
8   ASNTHRVALVALALAARGHISVALARGPRO
9   SERALASERILEPROVALVALPHESERARG
10   ASPGLYVALVALMETSERALATHRILEVAL
11   VALGLYGLULEUGLU

Samples:

sample_1: entity, [U-13C; U-15N], 0.5 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker DMX 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts