BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25835

Title: NMR structure of OtTx1a - AMP in DPC micelles

Deposition date: 2015-10-05 Original release date: 2016-09-29

Authors: Nadezhdin, Kirill; Romanovskaya, Daria; Sachkova, Maria; Vassilevski, Alexander; Grishin, Evgeny; Kovalchuk, Sergey; Arseniev, Alexander

Citation: Nadezhdin, Kirill; Romanovskaya, Daria; Sachkova, Maria; Vassilevski, Alexander; Grishin, Evgeny; Kovalchuk, Sergey; Arseniev, Alexander. "NMR structure of OtTx1a - AMP in DPC micelles"  Not known ., .-..

Assembly members:
entity, polymer, 41 residues, 4832.061 Da.

Natural source:   Common Name: spiders   Taxonomy ID: 666126   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oxyopes Takobius

Experimental source:   Production method: chemical synthesis   Host organism: none

Entity Sequences (FASTA):
entity: KFKWGKLFSTAKKLYKKGKK LSKNKNFKKALKFGKQLAKN L

Data sets:
Data typeCount
13C chemical shifts125
1H chemical shifts319

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 41 residues - 4832.061 Da.

1   LYSPHELYSTRPGLYLYSLEUPHESERTHR
2   ALALYSLYSLEUTYRLYSLYSGLYLYSLYS
3   LEUSERLYSASNLYSASNPHELYSLYSALA
4   LEULYSPHEGLYLYSGLNLEUALALYSASN
5   LEU

Samples:

sample_1: entity 0.6 mM; DPC 60 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 3.3; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

Molmol, Koradi, Billeter and Wuthrich - structure solution

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CARA, Keller and Wuthrich - chemical shift assignment

TALOS, Cornilescu, Delaglio and Bax - angle prediction

TOPSPIN, Bruker Biospin - collection, data analysis, processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

UNP P86716