BMRB Entry 25858
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25858
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution Structure of Lacticin Q and Aureocin A53 PubMed: 26771761
Deposition date: 2015-10-22 Original release date: 2016-02-15
Authors: Acedo, Jeella; van Belkum, Marco; Lohans, Christopher; Towle, Kaitlyn; Miskolzie, Mark; Vederas, John
Citation: Acedo, Jeella; van Belkum, Marco; Lohans, Christopher; Towle, Kaitlyn; Miskolzie, Mark; Vederas, John. "Nuclear Magnetic Resonance Solution Structures of Lacticin Q and Aureocin A53 Reveal a Structural Motif Conserved among Leaderless Bacteriocins with Broad-Spectrum Activity" Biochemistry 55, 733-742 (2016).
Assembly members:
Lacticin_Q, polymer, 53 residues, 5906.144 Da.
Natural source: Common Name: Lactococcus lactis Taxonomy ID: 1358 Superkingdom: Bacteria Kingdom: Eubacteria Genus/species: Lactococcus lactis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Lacticin_Q: MAGFLKVVQLLAKYGSKAVQ
WAWANKGKILDWLNAGQAID
WVVSKIKQILGIK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 391 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Lacticin Q | 1 |
Entities:
Entity 1, Lacticin Q 53 residues - 5906.144 Da.
| 1 | MET | ALA | GLY | PHE | LEU | LYS | VAL | VAL | GLN | LEU | ||||
| 2 | LEU | ALA | LYS | TYR | GLY | SER | LYS | ALA | VAL | GLN | ||||
| 3 | TRP | ALA | TRP | ALA | ASN | LYS | GLY | LYS | ILE | LEU | ||||
| 4 | ASP | TRP | LEU | ASN | ALA | GLY | GLN | ALA | ILE | ASP | ||||
| 5 | TRP | VAL | VAL | SER | LYS | ILE | LYS | GLN | ILE | LEU | ||||
| 6 | GLY | ILE | LYS |
Samples:
sample_1: Lacticin Q 0.8 mM; DSS 0.01 % w/v; TFE, [U-99% 2H], 25%; H2O 75%
sample_conditions_1: ionic strength: 0 M; pH: 6; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution
NMR spectrometers:
- Varian vNMRJ 700 MHz