BMRB Entry 25927
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25927
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Title: Curli secretion specificity factor CsgE W48A/F79A mutant PubMed: 27298344
Deposition date: 2015-12-21 Original release date: 2016-06-13
Authors: Shu, Qin; Krezel, Andrzej; Frieden, Carl
Citation: Shu, Qin; Krezel, Andrzej; Hultgren, Scott; Frieden, Carl. "Solution NMR structure of CsgE: Structural insights into a chaperone and regulator protein important for functional amyloid formation" Proc. Natl. Acad. Sci. U.S.A. 113, 7130-7135 (2016).
Assembly members:
entity, polymer, 108 residues, 13021.44 Da.
Natural source: Common Name: enterobacteria Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: AVEVEVPGLLTDHTVSSIGH
DFYRAFSDKWESDYTGNLTI
NERPSARAGSWITITVNQDV
IFQTFLFPLKRDFEKTVVAA
LIQTEEALNRRQINQALLST
GDLAHDEF
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 470 |
15N chemical shifts | 116 |
1H chemical shifts | 747 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | entity | 1 |
Entities:
Entity 1, entity 108 residues - 13021.44 Da.
1 | ALA | VAL | GLU | VAL | GLU | VAL | PRO | GLY | LEU | LEU | ||||
2 | THR | ASP | HIS | THR | VAL | SER | SER | ILE | GLY | HIS | ||||
3 | ASP | PHE | TYR | ARG | ALA | PHE | SER | ASP | LYS | TRP | ||||
4 | GLU | SER | ASP | TYR | THR | GLY | ASN | LEU | THR | ILE | ||||
5 | ASN | GLU | ARG | PRO | SER | ALA | ARG | ALA | GLY | SER | ||||
6 | TRP | ILE | THR | ILE | THR | VAL | ASN | GLN | ASP | VAL | ||||
7 | ILE | PHE | GLN | THR | PHE | LEU | PHE | PRO | LEU | LYS | ||||
8 | ARG | ASP | PHE | GLU | LYS | THR | VAL | VAL | ALA | ALA | ||||
9 | LEU | ILE | GLN | THR | GLU | GLU | ALA | LEU | ASN | ARG | ||||
10 | ARG | GLN | ILE | ASN | GLN | ALA | LEU | LEU | SER | THR | ||||
11 | GLY | ASP | LEU | ALA | HIS | ASP | GLU | PHE |
Samples:
15N: entity, [U-100% 15N], 180 uM; potassium phosphate 10 mM; D2O, [U-100% 13C; U-100% 15N; U-80% 2H], 10%; H2O 90%
15N-13C: entity, [U-99% 13C; U-99% 15N], 180 uM; potassium phosphate 10 mM; D2O, [U-100% 13C; U-100% 15N; U-80% 2H], 10%; H2O 90%
sample_conditions_1: ionic strength: 10 mM; pH: 5.8; pressure: 1 atm; temperature: 273 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | 15N | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | 15N-13C | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aromatic | 15N-13C | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aliphatic | 15N-13C | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | 15N-13C | isotropic | sample_conditions_1 |
3D C(CO)NH | 15N-13C | isotropic | sample_conditions_1 |
3D HNCO | 15N-13C | isotropic | sample_conditions_1 |
3D HNCACB | 15N-13C | isotropic | sample_conditions_1 |
3D HN(CO)CA | 15N-13C | isotropic | sample_conditions_1 |
3D C(CO)NH | 15N-13C | isotropic | sample_conditions_1 |
3D H(CCO)NH | 15N-13C | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | 15N | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | 15N-13C | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aromatic | 15N-13C | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.2, Bruker Biospin - collection, data analysis, processing
CYANA v3, Guntert, Mumenthaler and Wuthrich - structure solution
SPARKY, Goddard - chemical shift assignment, peak picking
Amber v12, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker DMX 750 MHz
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts