BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25949

Title: Ex-4[1-27]/pl14a

Deposition date: 2016-01-12 Original release date: 2016-05-23

Authors: Schroeder, Christina; Swedberg, Joakim; Craik, David

Citation: Swedberg, Joakim; Schroeder, Christina; Mitchell, Justin; Fairlie, David; Price, David; Liras, Spiros; Craik, David. "GLP-1 and exendin-4 alpha-conotoxin pl14a chimeras as potent GLP-1R agonists"  Not known ., .-..

Assembly members:
entity, polymer, 39 residues, 4496.178 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: HGEGTFTSDLSKQMEEEAVR CFIECLKGIGHKYPFCHCR

Data sets:
Data typeCount
1H chemical shifts242

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 39 residues - 4496.178 Da.

1   HISGLYGLUGLYTHRPHETHRSERASPLEU
2   SERLYSGLNMETGLUGLUGLUALAVALARG
3   CYSPHEILEGLUCYSLEULYSGLYILEGLY
4   HISLYSTYRPROPHECYSHISCYSARG

Samples:

sample_1: entity 0.2 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 3.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

Molmol, Koradi, Billeter and Wuthrich - data analysis

TOPSPIN, Bruker Biospin - collection

XEASY, Bartels et al. - chemical shift assignment, chemical shift calculation, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz