BMRB Entry 26058
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26058
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for a 58 truncated variant of the CI repressor from the temperated bacteriophage Tp901-1 PubMed: 27403839
Deposition date: 2016-05-18 Original release date: 2016-06-21
Authors: Krighaar Rasmussen, Kim; Ringkjobing-Jensen, Malene
Citation: Rasmussen, Kim; Frandsen, Kristian; Erba, Elisabetta; Pedersen, Margit; Varming, Anders; Hammer, Karin; Kilstrup, Mogens; Thulstrup, Peter; Blackledge, Martin; Jensen, Malene; Leggio, Leila. "Structural and dynamics studies of a truncated variant of CI repressor from bacteriophage TP901-1" Sci. Rep. 6, 29574-29574 (2016).
Assembly members:
d58CI, polymer, 130 residues, Formula weight is not available
Natural source: Common Name: Lactococcus phage TP901-1 Taxonomy ID: 35345 Superkingdom: Viruses Kingdom: not available Genus/species: bacteriophage TP901-1
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
d58CI: MQTDTSNRLKQIMAERNLKQ
VDILNLSIPFQKKFGIKLSK
STLSQYVNSVQSPDQNRIYL
LAKTLGVSEAWLMGFDVPMV
ESSKIENDSENIEETITVMK
KLEEPRQKVVLDTAKIQLKE
QDRSHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 246 |
| 15N chemical shifts | 117 |
| 1H chemical shifts | 117 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | dimer d58CI, chain 1 | 1 |
| 2 | dimer d58CI, chain 2 | 1 |
Entities:
Entity 1, dimer d58CI, chain 1 130 residues - Formula weight is not available
| 1 | MET | GLN | THR | ASP | THR | SER | ASN | ARG | LEU | LYS | |
| 2 | GLN | ILE | MET | ALA | GLU | ARG | ASN | LEU | LYS | GLN | |
| 3 | VAL | ASP | ILE | LEU | ASN | LEU | SER | ILE | PRO | PHE | |
| 4 | GLN | LYS | LYS | PHE | GLY | ILE | LYS | LEU | SER | LYS | |
| 5 | SER | THR | LEU | SER | GLN | TYR | VAL | ASN | SER | VAL | |
| 6 | GLN | SER | PRO | ASP | GLN | ASN | ARG | ILE | TYR | LEU | |
| 7 | LEU | ALA | LYS | THR | LEU | GLY | VAL | SER | GLU | ALA | |
| 8 | TRP | LEU | MET | GLY | PHE | ASP | VAL | PRO | MET | VAL | |
| 9 | GLU | SER | SER | LYS | ILE | GLU | ASN | ASP | SER | GLU | |
| 10 | ASN | ILE | GLU | GLU | THR | ILE | THR | VAL | MET | LYS | |
| 11 | LYS | LEU | GLU | GLU | PRO | ARG | GLN | LYS | VAL | VAL | |
| 12 | LEU | ASP | THR | ALA | LYS | ILE | GLN | LEU | LYS | GLU | |
| 13 | GLN | ASP | ARG | SER | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: d58CI, [U-13C; U-15N], 500 uM; TRIS 20 mM; NaCl 100 mM
sample_conditions_1: ionic strength: 120 mM; pH: 6.5; pressure: 1 atm; temperature: 311 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Varian Unity 600 MHz
- Varian Unity 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts