BMRB Entry 26562

Name: NMR assignments of the dust mite allergen Der p 23 from Dermatophagoides pteronyssinus
Published: 2016-01-06

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26562

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: NMR assignments of the dust mite allergen Der p 23 from Dermatophagoides pteronyssinus PubMed: 26602749

Deposition date: 2015-04-16 Original release date: 2016-01-06

Authors: Mueller, Geoffrey; London, Robert

Citation: Mueller, Geoffrey; London, Robert. "Serological, genomic, and structural analyses of the major mite allergen Der p 23" Clin. Exp. Allergy 46, 365-376 (2015).

Assembly members:
Der_p_23, polymer, 50 residues, Formula weight is not available

Natural source: Common Name: European house dust mite Taxonomy ID: 6956 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Dermatophagoides pteronyssinus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Der_p_23: SFTKFECPSRFGYFADPKDP HKFYICSNWEAVHKDCPGNT RWNEDEETCT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	139
15N chemical shifts	41
1H chemical shifts	207

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	major mite allergen Der p 23	1

Entities:

Entity 1, major mite allergen Der p 23 50 residues - Formula weight is not available

1	SER	PHE	THR	LYS	PHE	GLU	CYS	PRO	SER	ARG
2	PHE	GLY	TYR	PHE	ALA	ASP	PRO	LYS	ASP	PRO
3	HIS	LYS	PHE	TYR	ILE	CYS	SER	ASN	TRP	GLU
4	ALA	VAL	HIS	LYS	ASP	CYS	PRO	GLY	ASN	THR
5	ARG	TRP	ASN	GLU	ASP	GLU	GLU	THR	CYS	THR

Samples:

sample_1: Der p 23, [U-100% 13C; U-100% 15N], 0.5 ± 0.2 mM; sodium chloride 68.5 mM; potassium chloride 1.35 mM; sodium phosphate 5 mM; potassium phosphate 0.9 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.10185 M; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
hbcbcgcdhd	sample_1	isotropic	sample_conditions_1
hbcbcgcdcehe	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 600 MHz

Biological Magnetic Resonance Data Bank