BMRB Entry 26565
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26565
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for human Notch1 Transmembrane Domain Segment. PubMed: 26023825
Deposition date: 2015-04-21 Original release date: 2016-01-06
Authors: Deatherage, Catherine; Lu, Zhenwei; Kim, Ji-Hun; Sanders, Charles
Citation: Deatherage, Catherine; Lu, Zhenwei; Kim, Ji-Hun; Sanders, Charles. "Notch Transmembrane Domain: Secondary Structure and Topology" Biochemistry 54, 3565-3568 (2015).
Assembly members:
Notch1, polymer, 59 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Notch1: MGHHHHHHVQSETVEPPPPA
QLHFMYVAAAAFVLLFFVGC
GVLLSRKRRRQHGQLWFPE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 137 |
| 15N chemical shifts | 46 |
| 1H chemical shifts | 46 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Notch1 transmembrane segement | 1 |
Entities:
Entity 1, Notch1 transmembrane segement 59 residues - Formula weight is not available
Residues 1-8 represent a non-native his tag. This is the transmembrane segment of the human Notch1 receptor. As such, residue V9 is analogous to V1721 in the full length Notch 1 Protein.
| 1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | VAL | GLN | ||||
| 2 | SER | GLU | THR | VAL | GLU | PRO | PRO | PRO | PRO | ALA | ||||
| 3 | GLN | LEU | HIS | PHE | MET | TYR | VAL | ALA | ALA | ALA | ||||
| 4 | ALA | PHE | VAL | LEU | LEU | PHE | PHE | VAL | GLY | CYS | ||||
| 5 | GLY | VAL | LEU | LEU | SER | ARG | LYS | ARG | ARG | ARG | ||||
| 6 | GLN | HIS | GLY | GLN | LEU | TRP | PHE | PRO | GLU |
Samples:
sample_1: Notch1 Transmembrane segment, [U-98% 13C; U-98% 15N], 0.5 mM; sodium phosphate 20 mM; imidazole 500 mM; DHPC 65 mM; DMPC 195 mM; DTT 2 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker Avance 900 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts