BMRB Entry 26571
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26571
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: 1H, 13C and 15N NMR assignments of a calcium-binding protein(EhCaBP6) from Entamoeba histolytica PubMed: 26377206
Deposition date: 2015-05-06 Original release date: 2015-09-28
Authors: Verma, Deepshikha; Chary, Kandala
Citation: Verma, Deepshikha; Bhattacharya, Alok; Chary, Kandala. "1H, 13C and 15N NMR assignments of a calcium-binding protein from Entamoeba histolytica" Biomol. NMR Assignments 10, 67-70 (2016).
Assembly members:
EhCaBP6, polymer, 156 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.
Natural source: Common Name: Entamoeba histolytica Taxonomy ID: 5759 Superkingdom: Eukaryota Kingdom: not available Genus/species: Entamoeba histolytica
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
EhCaBP6: MSMEIEAPNANTQKIRDCFN
FYDRDYDGKIDVKQLGTLIR
SLGCAPTEDEVNSYIKEFAI
EGETFQIEQFELIMEREQSK
PDTREIKLRKAFEVFDQDKD
GKIKASDLAHNLTTVGDKMT
KEEVEKVFSILGITMESDID
LATFLKLVALHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 306 |
| 15N chemical shifts | 144 |
| 1H chemical shifts | 613 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EhCaBP6 | 1 |
| 2 | CALCIUM ION | 2 |
Entities:
Entity 1, EhCaBP6 156 residues - Formula weight is not available
| 1 | MET | SER | MET | GLU | ILE | GLU | ALA | PRO | ASN | ALA | ||||
| 2 | ASN | THR | GLN | LYS | ILE | ARG | ASP | CYS | PHE | ASN | ||||
| 3 | PHE | TYR | ASP | ARG | ASP | TYR | ASP | GLY | LYS | ILE | ||||
| 4 | ASP | VAL | LYS | GLN | LEU | GLY | THR | LEU | ILE | ARG | ||||
| 5 | SER | LEU | GLY | CYS | ALA | PRO | THR | GLU | ASP | GLU | ||||
| 6 | VAL | ASN | SER | TYR | ILE | LYS | GLU | PHE | ALA | ILE | ||||
| 7 | GLU | GLY | GLU | THR | PHE | GLN | ILE | GLU | GLN | PHE | ||||
| 8 | GLU | LEU | ILE | MET | GLU | ARG | GLU | GLN | SER | LYS | ||||
| 9 | PRO | ASP | THR | ARG | GLU | ILE | LYS | LEU | ARG | LYS | ||||
| 10 | ALA | PHE | GLU | VAL | PHE | ASP | GLN | ASP | LYS | ASP | ||||
| 11 | GLY | LYS | ILE | LYS | ALA | SER | ASP | LEU | ALA | HIS | ||||
| 12 | ASN | LEU | THR | THR | VAL | GLY | ASP | LYS | MET | THR | ||||
| 13 | LYS | GLU | GLU | VAL | GLU | LYS | VAL | PHE | SER | ILE | ||||
| 14 | LEU | GLY | ILE | THR | MET | GLU | SER | ASP | ILE | ASP | ||||
| 15 | LEU | ALA | THR | PHE | LEU | LYS | LEU | VAL | ALA | LEU | ||||
| 16 | HIS | HIS | HIS | HIS | HIS | HIS |
Entity 2, CALCIUM ION - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: EhCaBP6, [U-100% 13C], 1 mM; Tris.HCl 50 mM; NaCl 50 mM; CaCl2 4 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.1, BRUKER - processing
NMR spectrometers:
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts