BMRB Entry 26579
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26579
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Title: Resonance assignment of the N-terminal domain of hLCN2-R PubMed: 26635366
Deposition date: 2015-06-01 Original release date: 2016-10-04
Authors: Coudevylle, Nicolas; Zerko, Szymon; Kozminski, Wiktor; Konrat, Robert
Citation: Cabedo Martinez, Ana Isabel; Weinhaupl, Katharina; Lee, Wing-Kee; Wolff, Natascha; Storch, Barbara; Zerko, Szymon; Konrat, Robert; Kozminski, Wiktor; Breuker, Kathrin; Thevenod, Frank; Coudevylle, Nicolas. "Biochemical and Structural Characterization of the Interaction between the Siderocalin NGAL/LCN2 (Neutrophil Gelatinase-associated Lipocalin/Lipocalin 2) and the N-terminal Domain of Its Endocytic Receptor SLC22A17" J. Biol. Chem. 291, 2917-2930 (2016).
Assembly members:
hLCN2-R-NTD, polymer, 105 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
hLCN2-R-NTD: MASDPIFTLAPPLHCHYGAF
PPNASGWEQPPNASGVSVAS
AALAASAASRVATSTDPSCS
GFAPPDFNHCLKDWDYNGLP
VLTTNAIGQWDLVCDLGWQV
ILEQI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 255 |
| 15N chemical shifts | 88 |
| 1H chemical shifts | 342 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hLCN2-R-NTD | 1 |
Entities:
Entity 1, hLCN2-R-NTD 105 residues - Formula weight is not available
| 1 | MET | ALA | SER | ASP | PRO | ILE | PHE | THR | LEU | ALA | ||||
| 2 | PRO | PRO | LEU | HIS | CYS | HIS | TYR | GLY | ALA | PHE | ||||
| 3 | PRO | PRO | ASN | ALA | SER | GLY | TRP | GLU | GLN | PRO | ||||
| 4 | PRO | ASN | ALA | SER | GLY | VAL | SER | VAL | ALA | SER | ||||
| 5 | ALA | ALA | LEU | ALA | ALA | SER | ALA | ALA | SER | ARG | ||||
| 6 | VAL | ALA | THR | SER | THR | ASP | PRO | SER | CYS | SER | ||||
| 7 | GLY | PHE | ALA | PRO | PRO | ASP | PHE | ASN | HIS | CYS | ||||
| 8 | LEU | LYS | ASP | TRP | ASP | TYR | ASN | GLY | LEU | PRO | ||||
| 9 | VAL | LEU | THR | THR | ASN | ALA | ILE | GLY | GLN | TRP | ||||
| 10 | ASP | LEU | VAL | CYS | ASP | LEU | GLY | TRP | GLN | VAL | ||||
| 11 | ILE | LEU | GLU | GLN | ILE |
Samples:
sample_1: hLCN2-R-NTD, [U-95% 13C; U-95% 15N], 0.5 mM; H20 95%; D20 5%
sample_conditions_1: ionic strength: 70 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 5D HN(CA)CONH | sample_1 | isotropic | sample_conditions_1 |
| 5D (HACA)CON(CA)CONH | sample_1 | isotropic | sample_conditions_1 |
| 5D (H)NCO(NCA)CONH | sample_1 | isotropic | sample_conditions_1 |
| 4D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 5D HabCabCONH | sample_1 | isotropic | sample_conditions_1 |
| 5D H(CC-tocsy)CONH | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Varian INOVA 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts