BMRB Entry 26591
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26591
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Title: NMR Characterization of the Type III Secretion System Tip Chaperone Protein PcrG of Pseudomonas aeruginosa PubMed: 26259880
Deposition date: 2015-06-22 Original release date: 2015-12-18
Authors: Kaur, Kawaljit; De Guzman, Roberto
Citation: Chaudhury, Sukanya; Nordhues, Bryce; Kaur, Kawaljit; Zhang, Na; De Guzman, Roberto. "The LcrG Tip Chaperone Protein of the Yersinia pestis Type III Secretion System Is Partially Folded" J. Mol. Biol. 427, 3096-3109 (2015).
Assembly members:
PcrG9-76, polymer, 76 residues, Formula weight is not available
Natural source: Common Name: g-proteobacteria Taxonomy ID: 208964 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PcrG9-76: EDTLRATVQAAELAIRDSEE
RGRLLAEMWQGLGLAADAGE
LLFQAPERELARAAEEELLA
ELRRMRSSGSENLYFQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 145 |
| 15N chemical shifts | 75 |
| 1H chemical shifts | 74 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PcrG9-76 | 1 |
Entities:
Entity 1, PcrG9-76 76 residues - Formula weight is not available
Residues 77-84 corresponds to cloning artifact
| 1 | GLU | ASP | THR | LEU | ARG | ALA | THR | VAL | GLN | ALA | ||||
| 2 | ALA | GLU | LEU | ALA | ILE | ARG | ASP | SER | GLU | GLU | ||||
| 3 | ARG | GLY | ARG | LEU | LEU | ALA | GLU | MET | TRP | GLN | ||||
| 4 | GLY | LEU | GLY | LEU | ALA | ALA | ASP | ALA | GLY | GLU | ||||
| 5 | LEU | LEU | PHE | GLN | ALA | PRO | GLU | ARG | GLU | LEU | ||||
| 6 | ALA | ARG | ALA | ALA | GLU | GLU | GLU | LEU | LEU | ALA | ||||
| 7 | GLU | LEU | ARG | ARG | MET | ARG | SER | SER | GLY | SER | ||||
| 8 | GLU | ASN | LEU | TYR | PHE | GLN |
Samples:
sample_1: PcrG, [U-99% 13C; U-99% 15N], 0.7 mM; sodium chloride mM; sodium phosphate mM; D2O 10 mM; H2O 90 mM
sample_conditions_1: ionic strength: 10 mM; pH: 7; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts