BMRB Entry 26594
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26594
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Title: Backbone 15N, 1HN, 13CA, 13CB and 13C' Chemical shift Assignments for the DNA-binding domain of Human Telomeric Repeat Binding Factor Protein (hTRF1) PubMed: 26240333
Deposition date: 2015-06-26 Original release date: 2015-07-29
Authors: Sekhar, Ashok; Rosenzweig, Rina; Bouvignies, Guillaume; Kay, Lewis
Citation: Sekhar, Ashok; Rosenzweig, Rina; Bouvignies, Guillaume; Kay, Lewis. "Mapping the conformation of a client protein through the Hsp70 functional cycle" Proc. Natl. Acad. Sci. U.S.A. 112, 10395-10400 (2015).
Assembly members:
hTRF1, polymer, 53 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
hTRF1: RKRQAWLWEEDKNLRSGVRK
YGEGNWSKILLHYKFNNRTS
VMLKDRWRTMKKL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 141 |
| 15N chemical shifts | 48 |
| 1H chemical shifts | 48 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hTRF1 | 1 |
Entities:
Entity 1, hTRF1 53 residues - Formula weight is not available
| 1 | ARG | LYS | ARG | GLN | ALA | TRP | LEU | TRP | GLU | GLU | ||||
| 2 | ASP | LYS | ASN | LEU | ARG | SER | GLY | VAL | ARG | LYS | ||||
| 3 | TYR | GLY | GLU | GLY | ASN | TRP | SER | LYS | ILE | LEU | ||||
| 4 | LEU | HIS | TYR | LYS | PHE | ASN | ASN | ARG | THR | SER | ||||
| 5 | VAL | MET | LEU | LYS | ASP | ARG | TRP | ARG | THR | MET | ||||
| 6 | LYS | LYS | LEU |
Samples:
sample_1: hTRF1, [U-99% 13C; U-99% 15N], 0.8 mM; sodium acetate 50 mM; sodium chloride 50 mM; sodium azide 1 mM; EDTA 1 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.1 M; pH: 5.7; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Varian INOVA 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts