BMRB Entry 26595
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26595
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Title: Backbone 1H, 13C and 15N chemical shift assignments for hTRF1 bound to ADP-DnaK PubMed: 26240333
Deposition date: 2015-06-30 Original release date: 2015-07-29
Authors: Sekhar, Ashok; Rosenzweig, Rina; Bouvignies, Guillaume; Kay, Lewis
Citation: Sekhar, Ashok; Rosenzweig, Rina; Bouvignies, Guillaume; Kay, Lewis. "Mapping the conformation of a client protein through the Hsp70 functional cycle" Proc. Natl. Acad. Sci. U.S.A. 112, 10395-10400 (2015).
Assembly members:
hTRF1, polymer, 53 residues, Formula weight is not available
DnaK, polymer, 638 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
hTRF1: RKRQAWLWEEDKNLRSGVRK
YGEGNWSKILLHYKFNNRTS
VMLKDRWRTMKKL
DnaK: MGKIIGIDLGTTNSCVAIMD
GTTPRVLENAEGDRTTPSII
AYTQDGETLVGQPAKRQAVT
NPQNTLFAIKRLIGRRFQDE
EVQRDVSIMPFKIIAADNGD
AWVEVKGQKMAPPQISAEVL
KKMKKTAEDYLGEPVTEAVI
TVPAYFNDAQRQATKDAGRI
AGLEVKRIINEPTAAALAYG
LDKGTGNRTIAVYDLGGGTF
DISIIEIDEVDGEKTFEVLA
TNGDTHLGGEDFDSRLINYL
VEEFKKDQGIDLRNDPLAMQ
RLKEAAEKAKIELSSAQQTD
VNLPYITADATGPKHMNIKV
TRAKLESLVEDLVNRSIEPL
KVALQDAGLSVSDIDDVILV
GGQTRMPMVQKKVAEFFGKE
PRKDVNPDEAVAIGAAVQGG
VLTGDVKDVLLLDVTPLSLG
IETMGGVMTTLIAKNTTIPT
KHSQVFSTAEDNQSAVTIHV
LQGERKRAADNKSLGQFNLD
GINPAPRGMPQIEVTFDIDA
DGILHVSAKDKNSGKEQKIT
IKASSGLNEDEIQKMVRDAE
ANAEADRKFEELVQTRNQGD
HLLHSTRKQVEEAGDKLPAD
DKTAIESALTALETALKGED
KAAIEAKMQELAQVSQKLME
IAQQQHAQQQTAGADASANN
AKDDDVVDAEFEEVKDKK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 120 |
15N chemical shifts | 42 |
1H chemical shifts | 42 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | hTRF1 | 1 |
2 | DnaK | 2 |
Entities:
Entity 1, hTRF1 53 residues - Formula weight is not available
1 | ARG | LYS | ARG | GLN | ALA | TRP | LEU | TRP | GLU | GLU | ||||
2 | ASP | LYS | ASN | LEU | ARG | SER | GLY | VAL | ARG | LYS | ||||
3 | TYR | GLY | GLU | GLY | ASN | TRP | SER | LYS | ILE | LEU | ||||
4 | LEU | HIS | TYR | LYS | PHE | ASN | ASN | ARG | THR | SER | ||||
5 | VAL | MET | LEU | LYS | ASP | ARG | TRP | ARG | THR | MET | ||||
6 | LYS | LYS | LEU |
Entity 2, DnaK 638 residues - Formula weight is not available
1 | MET | GLY | LYS | ILE | ILE | GLY | ILE | ASP | LEU | GLY | ||||
2 | THR | THR | ASN | SER | CYS | VAL | ALA | ILE | MET | ASP | ||||
3 | GLY | THR | THR | PRO | ARG | VAL | LEU | GLU | ASN | ALA | ||||
4 | GLU | GLY | ASP | ARG | THR | THR | PRO | SER | ILE | ILE | ||||
5 | ALA | TYR | THR | GLN | ASP | GLY | GLU | THR | LEU | VAL | ||||
6 | GLY | GLN | PRO | ALA | LYS | ARG | GLN | ALA | VAL | THR | ||||
7 | ASN | PRO | GLN | ASN | THR | LEU | PHE | ALA | ILE | LYS | ||||
8 | ARG | LEU | ILE | GLY | ARG | ARG | PHE | GLN | ASP | GLU | ||||
9 | GLU | VAL | GLN | ARG | ASP | VAL | SER | ILE | MET | PRO | ||||
10 | PHE | LYS | ILE | ILE | ALA | ALA | ASP | ASN | GLY | ASP | ||||
11 | ALA | TRP | VAL | GLU | VAL | LYS | GLY | GLN | LYS | MET | ||||
12 | ALA | PRO | PRO | GLN | ILE | SER | ALA | GLU | VAL | LEU | ||||
13 | LYS | LYS | MET | LYS | LYS | THR | ALA | GLU | ASP | TYR | ||||
14 | LEU | GLY | GLU | PRO | VAL | THR | GLU | ALA | VAL | ILE | ||||
15 | THR | VAL | PRO | ALA | TYR | PHE | ASN | ASP | ALA | GLN | ||||
16 | ARG | GLN | ALA | THR | LYS | ASP | ALA | GLY | ARG | ILE | ||||
17 | ALA | GLY | LEU | GLU | VAL | LYS | ARG | ILE | ILE | ASN | ||||
18 | GLU | PRO | THR | ALA | ALA | ALA | LEU | ALA | TYR | GLY | ||||
19 | LEU | ASP | LYS | GLY | THR | GLY | ASN | ARG | THR | ILE | ||||
20 | ALA | VAL | TYR | ASP | LEU | GLY | GLY | GLY | THR | PHE | ||||
21 | ASP | ILE | SER | ILE | ILE | GLU | ILE | ASP | GLU | VAL | ||||
22 | ASP | GLY | GLU | LYS | THR | PHE | GLU | VAL | LEU | ALA | ||||
23 | THR | ASN | GLY | ASP | THR | HIS | LEU | GLY | GLY | GLU | ||||
24 | ASP | PHE | ASP | SER | ARG | LEU | ILE | ASN | TYR | LEU | ||||
25 | VAL | GLU | GLU | PHE | LYS | LYS | ASP | GLN | GLY | ILE | ||||
26 | ASP | LEU | ARG | ASN | ASP | PRO | LEU | ALA | MET | GLN | ||||
27 | ARG | LEU | LYS | GLU | ALA | ALA | GLU | LYS | ALA | LYS | ||||
28 | ILE | GLU | LEU | SER | SER | ALA | GLN | GLN | THR | ASP | ||||
29 | VAL | ASN | LEU | PRO | TYR | ILE | THR | ALA | ASP | ALA | ||||
30 | THR | GLY | PRO | LYS | HIS | MET | ASN | ILE | LYS | VAL | ||||
31 | THR | ARG | ALA | LYS | LEU | GLU | SER | LEU | VAL | GLU | ||||
32 | ASP | LEU | VAL | ASN | ARG | SER | ILE | GLU | PRO | LEU | ||||
33 | LYS | VAL | ALA | LEU | GLN | ASP | ALA | GLY | LEU | SER | ||||
34 | VAL | SER | ASP | ILE | ASP | ASP | VAL | ILE | LEU | VAL | ||||
35 | GLY | GLY | GLN | THR | ARG | MET | PRO | MET | VAL | GLN | ||||
36 | LYS | LYS | VAL | ALA | GLU | PHE | PHE | GLY | LYS | GLU | ||||
37 | PRO | ARG | LYS | ASP | VAL | ASN | PRO | ASP | GLU | ALA | ||||
38 | VAL | ALA | ILE | GLY | ALA | ALA | VAL | GLN | GLY | GLY | ||||
39 | VAL | LEU | THR | GLY | ASP | VAL | LYS | ASP | VAL | LEU | ||||
40 | LEU | LEU | ASP | VAL | THR | PRO | LEU | SER | LEU | GLY | ||||
41 | ILE | GLU | THR | MET | GLY | GLY | VAL | MET | THR | THR | ||||
42 | LEU | ILE | ALA | LYS | ASN | THR | THR | ILE | PRO | THR | ||||
43 | LYS | HIS | SER | GLN | VAL | PHE | SER | THR | ALA | GLU | ||||
44 | ASP | ASN | GLN | SER | ALA | VAL | THR | ILE | HIS | VAL | ||||
45 | LEU | GLN | GLY | GLU | ARG | LYS | ARG | ALA | ALA | ASP | ||||
46 | ASN | LYS | SER | LEU | GLY | GLN | PHE | ASN | LEU | ASP | ||||
47 | GLY | ILE | ASN | PRO | ALA | PRO | ARG | GLY | MET | PRO | ||||
48 | GLN | ILE | GLU | VAL | THR | PHE | ASP | ILE | ASP | ALA | ||||
49 | ASP | GLY | ILE | LEU | HIS | VAL | SER | ALA | LYS | ASP | ||||
50 | LYS | ASN | SER | GLY | LYS | GLU | GLN | LYS | ILE | THR | ||||
51 | ILE | LYS | ALA | SER | SER | GLY | LEU | ASN | GLU | ASP | ||||
52 | GLU | ILE | GLN | LYS | MET | VAL | ARG | ASP | ALA | GLU | ||||
53 | ALA | ASN | ALA | GLU | ALA | ASP | ARG | LYS | PHE | GLU | ||||
54 | GLU | LEU | VAL | GLN | THR | ARG | ASN | GLN | GLY | ASP | ||||
55 | HIS | LEU | LEU | HIS | SER | THR | ARG | LYS | GLN | VAL | ||||
56 | GLU | GLU | ALA | GLY | ASP | LYS | LEU | PRO | ALA | ASP | ||||
57 | ASP | LYS | THR | ALA | ILE | GLU | SER | ALA | LEU | THR | ||||
58 | ALA | LEU | GLU | THR | ALA | LEU | LYS | GLY | GLU | ASP | ||||
59 | LYS | ALA | ALA | ILE | GLU | ALA | LYS | MET | GLN | GLU | ||||
60 | LEU | ALA | GLN | VAL | SER | GLN | LYS | LEU | MET | GLU | ||||
61 | ILE | ALA | GLN | GLN | GLN | HIS | ALA | GLN | GLN | GLN | ||||
62 | THR | ALA | GLY | ALA | ASP | ALA | SER | ALA | ASN | ASN | ||||
63 | ALA | LYS | ASP | ASP | ASP | VAL | VAL | ASP | ALA | GLU | ||||
64 | PHE | GLU | GLU | VAL | LYS | ASP | LYS | LYS |
Samples:
sample_1: hTRF1, [U-13C; U-15N; U-2H], 0.5 mM; DnaK, [U-2H], 1.4 mM; MES 25 mM; KCl 25 mM; magnesium chloride 5 mM; adenosine diphosphate 5 mM; TCEP 1 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 6.0; pressure: 1 atm; temperature: 308 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts