BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26638

Title: Backbone and side chain chemical shift assignments of apolipophorin III from Galleria mellonella   PubMed: 26493308

Deposition date: 2015-08-20 Original release date: 2015-12-17

Authors: Crowhurst, Karin; Weers, Paul

Citation: Crowhurst, Karin; Horn, James; Weers, Paul. "Backbone and side chain chemical shift assignments of apolipophorin III from Galleria mellonella"  Biomol. NMR Assign. 10, 143-147 (2016).

Assembly members:
apolipophorin_III, polymer, 163 residues, Formula weight is not available

Natural source:   Common Name: greater wax moth   Taxonomy ID: 7137   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Galleria mellonella

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
apolipophorin_III: DASTPLQDLEKHAAEFQKTF SEQLNAFTNSKDTKEFNTAL KEGSDSVLQQLNALASSLQK ALNDANGKAKEALEQTRTNL ERTAEELRRAHPDVERQAGA LRDRLQTAVQATVQETQKLA KTVGANLEETNKKLAPQIKS AYDDFVKQAQEVQKKLHEAA SKQ

Data sets:
Data typeCount
13C chemical shifts625
15N chemical shifts185
1H chemical shifts828

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1apolipophorin III1

Entities:

Entity 1, apolipophorin III 163 residues - Formula weight is not available

1   ASPALASERTHRPROLEUGLNASPLEUGLU
2   LYSHISALAALAGLUPHEGLNLYSTHRPHE
3   SERGLUGLNLEUASNALAPHETHRASNSER
4   LYSASPTHRLYSGLUPHEASNTHRALALEU
5   LYSGLUGLYSERASPSERVALLEUGLNGLN
6   LEUASNALALEUALASERSERLEUGLNLYS
7   ALALEUASNASPALAASNGLYLYSALALYS
8   GLUALALEUGLUGLNTHRARGTHRASNLEU
9   GLUARGTHRALAGLUGLULEUARGARGALA
10   HISPROASPVALGLUARGGLNALAGLYALA
11   LEUARGASPARGLEUGLNTHRALAVALGLN
12   ALATHRVALGLNGLUTHRGLNLYSLEUALA
13   LYSTHRVALGLYALAASNLEUGLUGLUTHR
14   ASNLYSLYSLEUALAPROGLNILELYSSER
15   ALATYRASPASPPHEVALLYSGLNALAGLN
16   GLUVALGLNLYSLYSLEUHISGLUALAALA
17   SERLYSGLN

Samples:

apolipophorin_III_sample: apolipophorin III, [U-13C; U-15N], 1.2 mM; potassium phosphate 200 mM; sodium azide 2 mM; DSS 0.2 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0.3 M; pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCapolipophorin_III_sampleisotropicsample_conditions_1
2D 1H-15N HSQC NH2 onlyapolipophorin_III_sampleisotropicsample_conditions_1
2D 1H-13C HSQC aliphaticapolipophorin_III_sampleisotropicsample_conditions_1
2D 1H-13C HSQC aromaticapolipophorin_III_sampleisotropicsample_conditions_1
3D CBCA(CO)NHapolipophorin_III_sampleisotropicsample_conditions_1
3D C(CO)NHapolipophorin_III_sampleisotropicsample_conditions_1
3D HNCOapolipophorin_III_sampleisotropicsample_conditions_1
3D HNCACBapolipophorin_III_sampleisotropicsample_conditions_1
3D HBHA(CO)NHapolipophorin_III_sampleisotropicsample_conditions_1
3D HN(CO)CAapolipophorin_III_sampleisotropicsample_conditions_1
3D H(CCO)NHapolipophorin_III_sampleisotropicsample_conditions_1
3D HCCH-TOCSYapolipophorin_III_sampleisotropicsample_conditions_1
3D 1H-15N NOESYapolipophorin_III_sampleisotropicsample_conditions_1
3D 1H-13C NOESY aliphaticapolipophorin_III_sampleisotropicsample_conditions_1
3D 1H-13C NOESY aromaticapolipophorin_III_sampleisotropicsample_conditions_1
3D HCACOapolipophorin_III_sampleisotropicsample_conditions_1
3D HNHBapolipophorin_III_sampleisotropicsample_conditions_1
2D HbCbCgCdHdapolipophorin_III_sampleisotropicsample_conditions_1
2D HbCbCgCdCeHeapolipophorin_III_sampleisotropicsample_conditions_1

Software:

NMRPipe v8.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw v8.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v9.0.0-b114, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Agilent DD2 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts