BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26649

Title: 1H, 13C, 15N chemical shift assignments of initiation factor 1 from Pseudomonas aeruginosa   PubMed: 26983940

Deposition date: 2015-09-04 Original release date: 2016-08-22

Authors: Zhang, Yonghong

Citation: Bernal, Alejandra; Hu, Yanmei; Palmer, Stephanie; Silva, Aaron; Bullard, James; Zhang, Yonghong. "1H, 13C and 15N resonance assignments and secondary structure analysis of translation initiation factor 1 from Pseudomonas aeruginosa"  Biomol NMR Assign. 10, 249-252 (2016).

Assembly members:
Initiation_Factor_IF-1, polymer, 80 residues, Formula weight is not available

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Initiation_Factor_IF-1: MSKEDSFEMEGTVVDTLPNT MFRVELENGHVVTAHISGKM RKNYIRILTGDKVRVELTPY DLSKGRITYRARLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts269
15N chemical shifts70
1H chemical shifts460

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1IF11

Entities:

Entity 1, IF1 80 residues - Formula weight is not available

1   METSERLYSGLUASPSERPHEGLUMETGLU
2   GLYTHRVALVALASPTHRLEUPROASNTHR
3   METPHEARGVALGLULEUGLUASNGLYHIS
4   VALVALTHRALAHISILESERGLYLYSMET
5   ARGLYSASNTYRILEARGILELEUTHRGLY
6   ASPLYSVALARGVALGLULEUTHRPROTYR
7   ASPLEUSERLYSGLYARGILETHRTYRARG
8   ALAARGLEUGLUHISHISHISHISHISHIS

Samples:

sample_1: Initiation Factor IF-1, [U-100% 13C; U-100% 15N], 1 mM; KPO4 25 mM; KCl 100 mM

sample_conditions_1: ionic strength: 0.125 M; pH: 5.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts