BMRB Entry 26664
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26664
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Title: UBXD1 PubMed: 26475856
Deposition date: 2015-09-19 Original release date: 2015-11-09
Authors: Trusch, Franziska
Citation: Trusch, Franziska; Matena, Anja; Vuk, Maja; Koerver, Lisa; Knaevelsrud, Helene; Freemont, Paul; Meyer, Hemmo; Bayer, Peter. "The N-terminal Region of the UBX Domain-containing Protein 1 (UBXD1) Modulates Interdomain Communication within the Valosin-containing Protein p97" J. Biol. Chem. 290, 29414-29427 (2015).
Assembly members:
UBXD1-N, polymer, 120 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
UBXD1-N: MKKFFQEFKADIKFKSAGPG
QKLKESVGEKAHKEKPNQPA
PRPPRQGPTNEAQMAAAAAL
ARLEQKQSRAWGPTSQDTIR
NQVRKELQAEATVSGSPEAP
GTNVVSEPREEGSAHLAVPG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 196 |
15N chemical shifts | 91 |
1H chemical shifts | 178 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | UBXD1-N | 1 |
Entities:
Entity 1, UBXD1-N 120 residues - Formula weight is not available
1 | MET | LYS | LYS | PHE | PHE | GLN | GLU | PHE | LYS | ALA | |
2 | ASP | ILE | LYS | PHE | LYS | SER | ALA | GLY | PRO | GLY | |
3 | GLN | LYS | LEU | LYS | GLU | SER | VAL | GLY | GLU | LYS | |
4 | ALA | HIS | LYS | GLU | LYS | PRO | ASN | GLN | PRO | ALA | |
5 | PRO | ARG | PRO | PRO | ARG | GLN | GLY | PRO | THR | ASN | |
6 | GLU | ALA | GLN | MET | ALA | ALA | ALA | ALA | ALA | LEU | |
7 | ALA | ARG | LEU | GLU | GLN | LYS | GLN | SER | ARG | ALA | |
8 | TRP | GLY | PRO | THR | SER | GLN | ASP | THR | ILE | ARG | |
9 | ASN | GLN | VAL | ARG | LYS | GLU | LEU | GLN | ALA | GLU | |
10 | ALA | THR | VAL | SER | GLY | SER | PRO | GLU | ALA | PRO | |
11 | GLY | THR | ASN | VAL | VAL | SER | GLU | PRO | ARG | GLU | |
12 | GLU | GLY | SER | ALA | HIS | LEU | ALA | VAL | PRO | GLY |
Samples:
UBXD1-N_sample: UBXD1-N, [U-13C; U-15N], 250 uM; potassium phosphate buffer 50 mM; H2O 90%; D2O 10%
UBXD1-N_condition: ionic strength: 0.050 M; pH: 6.5; temperature: 273 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | UBXD1-N_sample | isotropic | UBXD1-N_condition |
2D 1H-1H NOESY | UBXD1-N_sample | isotropic | UBXD1-N_condition |
3D CBCA(CO)NH | UBXD1-N_sample | isotropic | UBXD1-N_condition |
3D HNCACB | UBXD1-N_sample | isotropic | UBXD1-N_condition |
3D HNCA | UBXD1-N_sample | isotropic | UBXD1-N_condition |
3D HNCO | UBXD1-N_sample | isotropic | UBXD1-N_condition |
3D HN(CO)CA | UBXD1-N_sample | isotropic | UBXD1-N_condition |
3D HCACO | UBXD1-N_sample | isotropic | UBXD1-N_condition |
3D 1H-15N TOCSY | UBXD1-N_sample | isotropic | UBXD1-N_condition |
3D 1H-15N NOESY | UBXD1-N_sample | isotropic | UBXD1-N_condition |
Software:
Auremol, Bruker Biospin - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts