BMRB Entry 26727
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26727
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Title: 1H, 13C and 15N assignment for the C-terminal domain of human Doublecortin PubMed: 27226599
Deposition date: 2016-01-10 Original release date: 2016-07-14
Authors: Di Lello, Paola
Citation: Burger, Dominique; Stihle, Martine; Sharma, Ashwani; Di Lello, Paola; Benz, Jorg; D'Arcy, Brigitte; Debulpaep, Maja; Fry, David; Huber, Walter; Kremer, Thomas; Laeremans, Toon; Matile, Hugues; Ross, Alfred; Rufer, Arne; Schoch, Guillaume; Steinmetz, Michel; Steyaert, Jan; Rudolph, Markus; Thoma, Ralf; Ruf, Armin. "Crystal Structures of the Human Doublecortin C- and N-terminal Domains in Complex with Specific Antibodies" J. Biol. Chem. 291, 16292-16306 (2016).
Assembly members:
Doublecortin_C-terminal_domain, polymer, 114 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Doublecortin_C-terminal_domain: LVPRGSHMARENKDFVRPKL
VTIIRSGVKPRKAVRVLLNK
KTAHSFEQVLTDITEAIKLE
TGVVKKLYTLDGKQVTCLHD
FFGDDDVFIACGPEKFRYAQ
DDFSLDENECRVMK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 417 |
15N chemical shifts | 108 |
1H chemical shifts | 109 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Doublecortin C-terminal domain monomer | 1 |
Entities:
Entity 1, Doublecortin C-terminal domain monomer 114 residues - Formula weight is not available
1 | LEU | VAL | PRO | ARG | GLY | SER | HIS | MET | ALA | ARG | ||||
2 | GLU | ASN | LYS | ASP | PHE | VAL | ARG | PRO | LYS | LEU | ||||
3 | VAL | THR | ILE | ILE | ARG | SER | GLY | VAL | LYS | PRO | ||||
4 | ARG | LYS | ALA | VAL | ARG | VAL | LEU | LEU | ASN | LYS | ||||
5 | LYS | THR | ALA | HIS | SER | PHE | GLU | GLN | VAL | LEU | ||||
6 | THR | ASP | ILE | THR | GLU | ALA | ILE | LYS | LEU | GLU | ||||
7 | THR | GLY | VAL | VAL | LYS | LYS | LEU | TYR | THR | LEU | ||||
8 | ASP | GLY | LYS | GLN | VAL | THR | CYS | LEU | HIS | ASP | ||||
9 | PHE | PHE | GLY | ASP | ASP | ASP | VAL | PHE | ILE | ALA | ||||
10 | CYS | GLY | PRO | GLU | LYS | PHE | ARG | TYR | ALA | GLN | ||||
11 | ASP | ASP | PHE | SER | LEU | ASP | GLU | ASN | GLU | CYS | ||||
12 | ARG | VAL | MET | LYS |
Samples:
sample_1: Doublecortin C-terminal domain, [U-99% 13C; U-99% 15N], 0.5 mM; sodium acetate 20 mM; TCEP 5 mM; D2O 5%; H2O 95%
sample_conditions_1: ionic strength: 20 mM; pH: 4.0; pressure: 1 atm; temperature: 300 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D (HB)CBCA(CO)NNH | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts