BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26755

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the intrinsically disordered NHE1 distal tail   PubMed: 27083547

Deposition date: 2016-03-11 Original release date: 2016-03-16

Authors: Hendus-Altenburger, Ruth; Kragelund, Birthe; Pedersen, Stine; Norholm, Ann-Beth

Citation: Hendus-Altenburger, Ruth; Pedraz-Cuesta, Elena; Olesen, Christina; Papaleo, Elena; Schnell, Jeff; Hopper, Jonathan; Robinson, Carol; Pedersen, Stine; Kragelund, Birthe. "The human Na(+)/H(+) exchanger 1 is a membrane scaffold protein for extracellular signal-regulated kinase 2"  BMC Biol. 14, .-. (2016).

Assembly members:
NHE1, polymer, 137 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
NHE1: MINNYLTVPAHKLDSPTMSR ARIGSDPLAYEPKEDLPVIT IDPASPQSPESVDLVNEELK GKVLGLSRDPAKVAEEDEDD DGGIMMRSKETSSPGTDDVF TPAPSDSPSSQRIQRCLSDP GPHPEPGEGEPFFPKGQ

Data sets:
Data typeCount
13C chemical shifts381
15N chemical shifts113
1H chemical shifts113

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NHE1, chain 11
2NHE1, chain 21

Entities:

Entity 1, NHE1, chain 1 137 residues - Formula weight is not available

Artificial Met at the beginning, I680-Q815 are from human NHE1 (SLC9A1)

1   METILEASNASNTYRLEUTHRVALPROALA
2   HISLYSLEUASPSERPROTHRMETSERARG
3   ALAARGILEGLYSERASPPROLEUALATYR
4   GLUPROLYSGLUASPLEUPROVALILETHR
5   ILEASPPROALASERPROGLNSERPROGLU
6   SERVALASPLEUVALASNGLUGLULEULYS
7   GLYLYSVALLEUGLYLEUSERARGASPPRO
8   ALALYSVALALAGLUGLUASPGLUASPASP
9   ASPGLYGLYILEMETMETARGSERLYSGLU
10   THRSERSERPROGLYTHRASPASPVALPHE
11   THRPROALAPROSERASPSERPROSERSER
12   GLNARGILEGLNARGCYSLEUSERASPPRO
13   GLYPROHISPROGLUPROGLYGLUGLYGLU
14   PROPHEPHEPROLYSGLYGLN

Samples:

NHE1cdt_WT: NHE1, [U-13C; U-15N], mM; sodium phosphate 10 mM; sodium chloride 137 mM; DSS 0.5 mM; DTT 10 mM; potassium chloride 2.7 mM; D2O, [U-100% 2H], 10 % v/v; potassium phosphate 1.8 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.2; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCNHE1cdt_WTisotropicsample_conditions_1
3D CBCA(CO)NHNHE1cdt_WTisotropicsample_conditions_1
3D HNCONHE1cdt_WTisotropicsample_conditions_1
3D HNCACBNHE1cdt_WTisotropicsample_conditions_1
3D HCACONHE1cdt_WTisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

NCBI P19634

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts