BMRB Entry 26759
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26759
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Title: Backbone resonance assignments for the SET domain of the human methyltransferase NSD2 PubMed: 27368234
Deposition date: 2016-03-17 Original release date: 2016-07-14
Authors: Bobby, Romel; Peciak, Karolina; Milbradt, Alexander
Citation: Bobby, Romel; Peciak, Karolina; Milbradt, Alexander. "Backbone resonance assignments for the SET domain of the human methyltransferase NSD2" Biomol. NMR Assign. 10, 307-310 (2016).
Assembly members:
NSD2_C16, polymer, 215 residues, 24308.6269 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
NSD2_C16: GSYKHIKVNKPYGKVQIYTA
DISEIPKCNCKPTDENPCGF
DSECLNRMLMFECHPQVCPA
GEFCQNQCFTKRQYPETKII
KTDGKGWGLVAKRDIRKGEF
VNEYVGELIDEEECMARIKH
AHENDITHFYMLTIDKDRII
DAGPKGNYSRFMNHSCQPNC
ETLKWTVNGDTRVGLFAVCD
IPAGTELTFNYNLDCLGNEK
TVCRCGASNCSGFLG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 584 |
15N chemical shifts | 189 |
1H chemical shifts | 189 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | NSD2 C16 | 1 |
Entities:
Entity 1, NSD2 C16 215 residues - 24308.6269 Da.
1 | GLY | SER | TYR | LYS | HIS | ILE | LYS | VAL | ASN | LYS | ||||
2 | PRO | TYR | GLY | LYS | VAL | GLN | ILE | TYR | THR | ALA | ||||
3 | ASP | ILE | SER | GLU | ILE | PRO | LYS | CYS | ASN | CYS | ||||
4 | LYS | PRO | THR | ASP | GLU | ASN | PRO | CYS | GLY | PHE | ||||
5 | ASP | SER | GLU | CYS | LEU | ASN | ARG | MET | LEU | MET | ||||
6 | PHE | GLU | CYS | HIS | PRO | GLN | VAL | CYS | PRO | ALA | ||||
7 | GLY | GLU | PHE | CYS | GLN | ASN | GLN | CYS | PHE | THR | ||||
8 | LYS | ARG | GLN | TYR | PRO | GLU | THR | LYS | ILE | ILE | ||||
9 | LYS | THR | ASP | GLY | LYS | GLY | TRP | GLY | LEU | VAL | ||||
10 | ALA | LYS | ARG | ASP | ILE | ARG | LYS | GLY | GLU | PHE | ||||
11 | VAL | ASN | GLU | TYR | VAL | GLY | GLU | LEU | ILE | ASP | ||||
12 | GLU | GLU | GLU | CYS | MET | ALA | ARG | ILE | LYS | HIS | ||||
13 | ALA | HIS | GLU | ASN | ASP | ILE | THR | HIS | PHE | TYR | ||||
14 | MET | LEU | THR | ILE | ASP | LYS | ASP | ARG | ILE | ILE | ||||
15 | ASP | ALA | GLY | PRO | LYS | GLY | ASN | TYR | SER | ARG | ||||
16 | PHE | MET | ASN | HIS | SER | CYS | GLN | PRO | ASN | CYS | ||||
17 | GLU | THR | LEU | LYS | TRP | THR | VAL | ASN | GLY | ASP | ||||
18 | THR | ARG | VAL | GLY | LEU | PHE | ALA | VAL | CYS | ASP | ||||
19 | ILE | PRO | ALA | GLY | THR | GLU | LEU | THR | PHE | ASN | ||||
20 | TYR | ASN | LEU | ASP | CYS | LEU | GLY | ASN | GLU | LYS | ||||
21 | THR | VAL | CYS | ARG | CYS | GLY | ALA | SER | ASN | CYS | ||||
22 | SER | GLY | PHE | LEU | GLY |
Samples:
sample_1: NSD2_C16, [U-13C; U-15N; U-2H], 0.6 mM; SAM 1.2 mM; NaCl 100.0 mM; TCEP 1.0 mM; NaN3 1.0%
sample_conditions_1: ionic strength: 0.140 M; pH: 7.500; pressure: 1.000 atm; temperature: 298.000 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC/HMQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
HNCOCACB (H[N[co[{CA|ca[C]}]]]) | sample_1 | isotropic | sample_conditions_1 |
HNCOCA (H[N[co[{CA|ca[C]}]]]) | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNmr_Analysis v2.1, CCPN - Spectrum display, Spectrum analysis
Topspin v3.2.5, Bruker Biospin - Spectrum acquisition, Spectrum display
nmrDraw vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum analysis, Spectrum display
nmrPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing
NMR spectrometers:
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts