BMRB Entry 26761
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26761
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Title: Backbone assignment of the Xylanase B2 from Streptomyces lividans PubMed: 27387012
Deposition date: 2016-03-19 Original release date: 2016-04-22
Authors: Gagne, Donald; Nguyen Thi, Nhung; Narayanan, Chitra; Roux, Louise; Brunzelle, Joseph; Couture, Jean-Francois; Agarwal, Pratul; Doucet, Nicolas
Citation: Gagne, Donald; Nguyen Thi, Nhung; Narayanan, Chitra; Roux, Louise; Brunzelle, Joseph; Couture, Jean-Francois; Agarwal, Pratul; Doucet, Nicolas. "Ligand Binding Enhances Millisecond Conformational Exchange in Xylanase B2 from Streptomyces lividans" Biochemistry 55, 4184-4196 (2016).
Assembly members:
XlnB2, polymer, 191 residues, Formula weight is not available
Natural source: Common Name: Streptomyces lividans Taxonomy ID: 1916 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces lividans
Experimental source: Production method: recombinant technology Host organism: Streptomyces lividans
Entity Sequences (FASTA):
XlnB2: DTVVTTNQEGTNNGYYYSFW
TDSQGTVSMNMGSGGQYSTS
WRNTGNFVAGKGWANGGRRT
VQYSGSFNPSGNAYLALYGW
TSNPLVEYYIVDNWGTYRPT
GEYKGTVTSDGGTYDIYKTT
RVNKPSVEGTRTFDQYWSVR
QSKRTGGTITTGNHFDAWAR
AGMPLGNFSYYMIMATEGYQ
SSGSSSINVGG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 446 |
| 15N chemical shifts | 147 |
| 1H chemical shifts | 146 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | XlnB2 | 1 |
Entities:
Entity 1, XlnB2 191 residues - Formula weight is not available
| 1 | ASP | THR | VAL | VAL | THR | THR | ASN | GLN | GLU | GLY | ||||
| 2 | THR | ASN | ASN | GLY | TYR | TYR | TYR | SER | PHE | TRP | ||||
| 3 | THR | ASP | SER | GLN | GLY | THR | VAL | SER | MET | ASN | ||||
| 4 | MET | GLY | SER | GLY | GLY | GLN | TYR | SER | THR | SER | ||||
| 5 | TRP | ARG | ASN | THR | GLY | ASN | PHE | VAL | ALA | GLY | ||||
| 6 | LYS | GLY | TRP | ALA | ASN | GLY | GLY | ARG | ARG | THR | ||||
| 7 | VAL | GLN | TYR | SER | GLY | SER | PHE | ASN | PRO | SER | ||||
| 8 | GLY | ASN | ALA | TYR | LEU | ALA | LEU | TYR | GLY | TRP | ||||
| 9 | THR | SER | ASN | PRO | LEU | VAL | GLU | TYR | TYR | ILE | ||||
| 10 | VAL | ASP | ASN | TRP | GLY | THR | TYR | ARG | PRO | THR | ||||
| 11 | GLY | GLU | TYR | LYS | GLY | THR | VAL | THR | SER | ASP | ||||
| 12 | GLY | GLY | THR | TYR | ASP | ILE | TYR | LYS | THR | THR | ||||
| 13 | ARG | VAL | ASN | LYS | PRO | SER | VAL | GLU | GLY | THR | ||||
| 14 | ARG | THR | PHE | ASP | GLN | TYR | TRP | SER | VAL | ARG | ||||
| 15 | GLN | SER | LYS | ARG | THR | GLY | GLY | THR | ILE | THR | ||||
| 16 | THR | GLY | ASN | HIS | PHE | ASP | ALA | TRP | ALA | ARG | ||||
| 17 | ALA | GLY | MET | PRO | LEU | GLY | ASN | PHE | SER | TYR | ||||
| 18 | TYR | MET | ILE | MET | ALA | THR | GLU | GLY | TYR | GLN | ||||
| 19 | SER | SER | GLY | SER | SER | SER | ILE | ASN | VAL | GLY | ||||
| 20 | GLY |
Samples:
sample_1: XlnB2, [U-100% 15N], 3 g/L; XlnB2, [U-100% 13C], 2 g/L
sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts