BMRB Entry 26768
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26768
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Title: 1H, 15N and 13C backbone assignments of the catalytic domain of human NSD2 PubMed: 27356987
Deposition date: 2016-03-23 Original release date: 2016-04-22
Authors: Amin, Nader; Nietlispach, Daniel; Coyle, Joe; Williams, Glyn; Chiarparin, Elisabetta
Citation: Amin, Nader; Nietlispach, Daniel; Qamar, Seema; Coyle, Joe; Chiarparin, Elisabetta; Williams, Glyn. "NMR backbone resonance assignment and solution secondary structure determination of human NSD1 and NSD2" Biomol. NMR Assign. 10, 315-320 (2016).
Assembly members:
NSD2, polymer, 235 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
NSD2: GSHMKLQREARETQESERKP
PPYKHIKVNKPYGKVQIYTA
DISEIPKCNCKPTDENPCGF
DSECLNRMLMFECHPQVCPA
GEFCQNQCFTKRQYPETKII
KTDGKGWGLVAKRDIRKGEF
VNEYVGELIDEEECMARIKH
AHENDITHFYMLTIDKDRII
DAGPKGNYSRFMNHSCQPNC
ETLKWTVNGDTRVGLFAVCD
IPAGTELTFNYNLDCLGNEK
TVCRCGASNCSGFLG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 629 |
15N chemical shifts | 206 |
1H chemical shifts | 206 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | NSD2 | 1 |
Entities:
Entity 1, NSD2 235 residues - Formula weight is not available
1 | GLY | SER | HIS | MET | LYS | LEU | GLN | ARG | GLU | ALA | ||||
2 | ARG | GLU | THR | GLN | GLU | SER | GLU | ARG | LYS | PRO | ||||
3 | PRO | PRO | TYR | LYS | HIS | ILE | LYS | VAL | ASN | LYS | ||||
4 | PRO | TYR | GLY | LYS | VAL | GLN | ILE | TYR | THR | ALA | ||||
5 | ASP | ILE | SER | GLU | ILE | PRO | LYS | CYS | ASN | CYS | ||||
6 | LYS | PRO | THR | ASP | GLU | ASN | PRO | CYS | GLY | PHE | ||||
7 | ASP | SER | GLU | CYS | LEU | ASN | ARG | MET | LEU | MET | ||||
8 | PHE | GLU | CYS | HIS | PRO | GLN | VAL | CYS | PRO | ALA | ||||
9 | GLY | GLU | PHE | CYS | GLN | ASN | GLN | CYS | PHE | THR | ||||
10 | LYS | ARG | GLN | TYR | PRO | GLU | THR | LYS | ILE | ILE | ||||
11 | LYS | THR | ASP | GLY | LYS | GLY | TRP | GLY | LEU | VAL | ||||
12 | ALA | LYS | ARG | ASP | ILE | ARG | LYS | GLY | GLU | PHE | ||||
13 | VAL | ASN | GLU | TYR | VAL | GLY | GLU | LEU | ILE | ASP | ||||
14 | GLU | GLU | GLU | CYS | MET | ALA | ARG | ILE | LYS | HIS | ||||
15 | ALA | HIS | GLU | ASN | ASP | ILE | THR | HIS | PHE | TYR | ||||
16 | MET | LEU | THR | ILE | ASP | LYS | ASP | ARG | ILE | ILE | ||||
17 | ASP | ALA | GLY | PRO | LYS | GLY | ASN | TYR | SER | ARG | ||||
18 | PHE | MET | ASN | HIS | SER | CYS | GLN | PRO | ASN | CYS | ||||
19 | GLU | THR | LEU | LYS | TRP | THR | VAL | ASN | GLY | ASP | ||||
20 | THR | ARG | VAL | GLY | LEU | PHE | ALA | VAL | CYS | ASP | ||||
21 | ILE | PRO | ALA | GLY | THR | GLU | LEU | THR | PHE | ASN | ||||
22 | TYR | ASN | LEU | ASP | CYS | LEU | GLY | ASN | GLU | LYS | ||||
23 | THR | VAL | CYS | ARG | CYS | GLY | ALA | SER | ASN | CYS | ||||
24 | SER | GLY | PHE | LEU | GLY |
Samples:
sample_1: NSD2, [U-98% 13C; U-98% 15N], 0.2 mM; potassium phosphate 50 mM; sodium chloride 450 mM; TCEP 2 mM; S-adenosyl methionine 2 mM; sodium azide 0.01 % w/v; D2O 10 % v/v
sample_conditions_1: ionic strength: 450 mM; pH: 7; pressure: 1 atm; temperature: 310 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CCPN_Analysis, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts