BMRB Entry 26792
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26792
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Title: Backbone assignment of p23 GOLD domain PubMed: 27569046
Deposition date: 2016-05-10 Original release date: 2016-10-14
Authors: Yamaguchi, Yoshiki; Nagae, Masamichi
Citation: Nagae, Masamichi; Hirata, Tetsuya; Morita-Matsumoto, Kana; Theiler, Romina; Fujita, Morihisa; Kinoshita, Taroh; Yamaguchi, Yoshiki. "3D structure and interaction of p24beta and p24delta Golgi dynamics domains: implications for p24 complex formation and cargo transport" J. Mol. Biol. 428, 4087-4099 (2016).
Assembly members:
p23, polymer, 103 residues, Formula weight is not available
Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
p23: GSISFHLPVNSRKCLREEIH
KDLLVTGAYEITDQSGGAGG
LRTHLKITDSAGHILYAKED
ATKGKFAFTTEDYDMFEVCF
ESKGTGRIPDQLVILDMKHG
VEA
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 162 |
15N chemical shifts | 81 |
1H chemical shifts | 81 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | GOLD domain | 1 |
Entities:
Entity 1, GOLD domain 103 residues - Formula weight is not available
1 | GLY | SER | ILE | SER | PHE | HIS | LEU | PRO | VAL | ASN | ||||
2 | SER | ARG | LYS | CYS | LEU | ARG | GLU | GLU | ILE | HIS | ||||
3 | LYS | ASP | LEU | LEU | VAL | THR | GLY | ALA | TYR | GLU | ||||
4 | ILE | THR | ASP | GLN | SER | GLY | GLY | ALA | GLY | GLY | ||||
5 | LEU | ARG | THR | HIS | LEU | LYS | ILE | THR | ASP | SER | ||||
6 | ALA | GLY | HIS | ILE | LEU | TYR | ALA | LYS | GLU | ASP | ||||
7 | ALA | THR | LYS | GLY | LYS | PHE | ALA | PHE | THR | THR | ||||
8 | GLU | ASP | TYR | ASP | MET | PHE | GLU | VAL | CYS | PHE | ||||
9 | GLU | SER | LYS | GLY | THR | GLY | ARG | ILE | PRO | ASP | ||||
10 | GLN | LEU | VAL | ILE | LEU | ASP | MET | LYS | HIS | GLY | ||||
11 | VAL | GLU | ALA |
Samples:
sample_1: p23, [U-99% 13C; U-99% 15N], 0.5 mM; H2O 90%; D2O 10%; sodium phosphate 20 mM; NaCl 50 mM
sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts