BMRB Entry 26803
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26803
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Title: Recombinant human carcinoembryonic antigen related cell adhesion molecule 1 (hCEACAM1) N-terminal Igv domain PubMed: 27471271
Deposition date: 2016-05-18 Original release date: 2016-08-31
Authors: Prestegard, James; Zhuo, You
Citation: Zhuo, You; Yang, Jeong-Yeh; Moremen, Kelley; Prestegard, James. "Glycosylation Alters Dimerization Properties of a Cell-surface Signaling Protein, Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM1)" J. Biol. Chem. 291, 20085-20095 (2016).
Assembly members:
CEACAM1-Igv, polymer, 120 residues, 13371.78 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CEACAM1-Igv: MAQLTTESMPFNVAEGKEVL
LLVHNLPQQLFGYSWYKGER
VDGNRQIVGYAIGTQQATPG
PANSGRETIYPNASLLIQNV
TQNDTGFYTLQVIKSDLVNE
EATGQFHVYAAALEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 247 |
| 15N chemical shifts | 83 |
| 1H chemical shifts | 83 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CEACAM1-Igv, chain 1 | 1 |
| 2 | CEACAM1-Igv, chain 2 | 1 |
Entities:
Entity 1, CEACAM1-Igv, chain 1 120 residues - 13371.78 Da.
Residues 110-120 represent a non-native His-tag.
| 1 | MET | ALA | GLN | LEU | THR | THR | GLU | SER | MET | PRO | |
| 2 | PHE | ASN | VAL | ALA | GLU | GLY | LYS | GLU | VAL | LEU | |
| 3 | LEU | LEU | VAL | HIS | ASN | LEU | PRO | GLN | GLN | LEU | |
| 4 | PHE | GLY | TYR | SER | TRP | TYR | LYS | GLY | GLU | ARG | |
| 5 | VAL | ASP | GLY | ASN | ARG | GLN | ILE | VAL | GLY | TYR | |
| 6 | ALA | ILE | GLY | THR | GLN | GLN | ALA | THR | PRO | GLY | |
| 7 | PRO | ALA | ASN | SER | GLY | ARG | GLU | THR | ILE | TYR | |
| 8 | PRO | ASN | ALA | SER | LEU | LEU | ILE | GLN | ASN | VAL | |
| 9 | THR | GLN | ASN | ASP | THR | GLY | PHE | TYR | THR | LEU | |
| 10 | GLN | VAL | ILE | LYS | SER | ASP | LEU | VAL | ASN | GLU | |
| 11 | GLU | ALA | THR | GLY | GLN | PHE | HIS | VAL | TYR | ALA | |
| 12 | ALA | ALA | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: CEACAM1-Igv, [U-98% 13C; U-98% 15N], 200 uM; TRIS 10 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.007 M; pH: 7.4; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts