BMRB Entry 26810

Name: Backbone Chemical Shift Assignment of LIN54 residues 586 to 646.
Published: 2016-08-31

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26810

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone Chemical Shift Assignment of LIN54 residues 586 to 646. PubMed: 27465258

Deposition date: 2016-05-31 Original release date: 2016-08-31

Authors: Marceau, Aimee; Lee, Hsiau-Wei; Rubin, Seth

Citation: Marceau, Aimee; Felthousen, Jessica; Goetsch, Paul; Iness, Audra; Lee, Hsiau-Wei; Tripathi, Sarvind; Strome, Susan; Litovchick, Larisa; Rubin, Seth. "Structural basis for LIN54 recognition of CHR elements in cell cycle-regulated promoters" Nat. Comm. 7, 12301-12301 (2016).

Assembly members:
LIN54, polymer, 61 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
LIN54: GEFGESDRRHSKGCNCKRSG CLKNYCECYEAKIMCSSICK CIGCKNFEESPERKTLMHLA D

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	176
15N chemical shifts	62
1H chemical shifts	65

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LIN54	1
2	ZINC ION, 1	2
3	ZINC ION, 2	2
4	ZINC ION, 3	2

Entities:

Entity 1, LIN54 61 residues - Formula weight is not available

1

GLY

GLU

PHE

GLY

GLU

SER

ASP

ARG

HIS

2

SER

LYS

GLY

CYS

ASN

CYS

LYS

ARG

SER

GLY

3

CYS

LEU

LYS

ASN

TYR

CYS

GLU

CYS

TYR

GLU

4

ALA

LYS

ILE

MET

CYS

SER

ILE

CYS

LYS

5

CYS

ILE

GLY

CYS

LYS

ASN

PHE

GLU

SER

6

PRO

GLU

ARG

LYS

THR

LEU

MET

HIS

LEU

ALA

7

ASP

Entity 2, ZINC ION, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: LIN54, [U-100% 13C; U-100% 15N], 0.4 mM; MES 35 mM; sodium chloride 75 mM

sample_conditions_1: ionic strength: 75 mM; pH: 6; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

Varian INOVA 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts