BMRB Entry 26820
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR26820
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Title: Lunasin assignment (truncated form 16-43) PubMed: 27639324
Deposition date: 2016-06-20 Original release date: 2016-07-20
Authors: Aleksis, Rihards; Jaudzems, Kristaps; Muceniece, Ruta; Liepinsh, Edvards
Citation: Aleksis, Rihards; Jaudzems, Kristaps; Muceniece, Ruta; Liepinsh, Edvards. "Lunasin is a redox sensitive intrinsically disordered peptide with two transiently populated \u03b1-helical regions" Peptides 85, 56-62 (2016).
Assembly members:
Lunasin16-43, polymer, 28 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Lunasin16-43: GVNLTPCEKHIMEKIQGRGD
DDDDDDDD
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 157 |
15N chemical shifts | 58 |
1H chemical shifts | 344 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Lunasin16-43 | 1 |
Entities:
Entity 1, Lunasin16-43 28 residues - Formula weight is not available
1 | GLY | VAL | ASN | LEU | THR | PRO | CYS | GLU | LYS | HIS | ||||
2 | ILE | MET | GLU | LYS | ILE | GLN | GLY | ARG | GLY | ASP | ||||
3 | ASP | ASP | ASP | ASP | ASP | ASP | ASP | ASP |
Samples:
sample_1: Lunasin16-43, [U-99% 15N], 1 mM; D2O, [U-99% 2H], 7%; H2O 93%; sodium phosphate 20 mM; sodium azide 0.03%
pH35: ionic strength: 20 mM; pH: 3.5; pressure: 1 atm; temperature: 273 K
pH65: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 273 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | pH35 |
2D 1H-1H TOCSY | sample_1 | isotropic | pH35 |
2D 1H-1H NOESY | sample_1 | isotropic | pH35 |
3D 1H-15N NOESY | sample_1 | isotropic | pH35 |
2D 1H-13C HSQC aliphatic | sample_1 | isotropic | pH35 |
2D 1H-15N HSQC | sample_1 | isotropic | pH65 |
2D 1H-1H TOCSY | sample_1 | isotropic | pH65 |
2D 1H-1H NOESY | sample_1 | isotropic | pH65 |
3D 1H-15N NOESY | sample_1 | isotropic | pH65 |
2D 1H-13C HSQC aliphatic | sample_1 | isotropic | pH65 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts