BMRB Entry 26852
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26852
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Title: Partial Backbone 1H, 13C, and 15N Chemical Shift Assignments for VirB8 from Brucella suis PubMed: 28236662
Deposition date: 2016-07-13 Original release date: 2017-02-22
Authors: Sharifahmadian, Mahzad; Lecoq, Lauriane; Omichinski, James; Baron, Christian
Citation: Sharifahmadian, Mahzad; Arya, Tarun; Bessette, Benoit; Lecoq, Lauriane; Ruediger, Edward; Omichinski, James; Baron, Christian. "Monomer-to-dimer transition of Brucella suis type IV secretion system component VirB8 induces conformational changes" FEBS J. 284, 1218-1232 (2017).
Assembly members:
VirB8p, polymer, 164 residues, 18202.1504 Da.
Natural source: Common Name: enterobacteria Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
VirB8p: ARVNAQTGAPDILTSLDEKS
VSYDTVMDKYWLSQYVIARE
TYDWYTLQKDYETVGMLSSP
SEGQSYASQFQGDKALDKQY
GSNVRTSVTIVSIVPNGKGI
GTVRFAKTTKRTNETGDGET
THWIATIGYQYVNPSLMSES
ARLTNPLGFNVTSYRVDPEM
GVVQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 416 |
| 15N chemical shifts | 146 |
| 1H chemical shifts | 146 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | VirB8p subunit, 1 | 1 |
| 2 | VirB8p subunit, 2 | 1 |
Entities:
Entity 1, VirB8p subunit, 1 164 residues - 18202.1504 Da.
| 1 | ALA | ARG | VAL | ASN | ALA | GLN | THR | GLY | ALA | PRO | ||||
| 2 | ASP | ILE | LEU | THR | SER | LEU | ASP | GLU | LYS | SER | ||||
| 3 | VAL | SER | TYR | ASP | THR | VAL | MET | ASP | LYS | TYR | ||||
| 4 | TRP | LEU | SER | GLN | TYR | VAL | ILE | ALA | ARG | GLU | ||||
| 5 | THR | TYR | ASP | TRP | TYR | THR | LEU | GLN | LYS | ASP | ||||
| 6 | TYR | GLU | THR | VAL | GLY | MET | LEU | SER | SER | PRO | ||||
| 7 | SER | GLU | GLY | GLN | SER | TYR | ALA | SER | GLN | PHE | ||||
| 8 | GLN | GLY | ASP | LYS | ALA | LEU | ASP | LYS | GLN | TYR | ||||
| 9 | GLY | SER | ASN | VAL | ARG | THR | SER | VAL | THR | ILE | ||||
| 10 | VAL | SER | ILE | VAL | PRO | ASN | GLY | LYS | GLY | ILE | ||||
| 11 | GLY | THR | VAL | ARG | PHE | ALA | LYS | THR | THR | LYS | ||||
| 12 | ARG | THR | ASN | GLU | THR | GLY | ASP | GLY | GLU | THR | ||||
| 13 | THR | HIS | TRP | ILE | ALA | THR | ILE | GLY | TYR | GLN | ||||
| 14 | TYR | VAL | ASN | PRO | SER | LEU | MET | SER | GLU | SER | ||||
| 15 | ALA | ARG | LEU | THR | ASN | PRO | LEU | GLY | PHE | ASN | ||||
| 16 | VAL | THR | SER | TYR | ARG | VAL | ASP | PRO | GLU | MET | ||||
| 17 | GLY | VAL | VAL | GLN |
Samples:
sample_1: Molecule_VirB8p, [U-13C; U-15N], 0.35 mM; sodium phosphate 20 mM; sodium chloride 100 mM; D2O, [U-99% 2H], 10%; H2O 90%; CHAPS 30 mM
sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNmr_Analysis v2.4, CCPN - chemical shift assignment, data analysis
NMR spectrometers:
- Varian INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts