BMRB Entry 26862
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26862
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Title: Stress Response Peptide-2; Manduca Sexta PubMed: 27903232
Deposition date: 2016-07-27 Original release date: 2017-02-16
Authors: Schrag, Lynn; Cao, Xiaolong; Herrera, Alvaro; Wang, Yang; Jiang, Haobo; Prakash, Om
Citation: Schrag, Lynn Goss; Cao, Xiaolong; Herrera, Alvaro Ivan; Wang, Yang; Jiang, Haobo; Prakash, Om. "Solution Structure and Expression Profile of an Insect Cytokine: Manduca sexta Stress Response Peptide-2" Protein Pept. Lett. 24, 3-11 (2017).
Assembly members:
Stress_Response_Peptide-2, polymer, 25 residues, 2751.12 Da.
Natural source: Common Name: tobacco hornworm Taxonomy ID: 7130 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Manduca Sexta
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Stress_Response_Peptide-2: FGVKDGKCPSGRVRRLGICV
PDDDY
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 148 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Stress Response Peptide-2 | 1 |
Entities:
Entity 1, Stress Response Peptide-2 25 residues - 2751.12 Da.
1 | PHE | GLY | VAL | LYS | ASP | GLY | LYS | CYS | PRO | SER | ||||
2 | GLY | ARG | VAL | ARG | ARG | LEU | GLY | ILE | CYS | VAL | ||||
3 | PRO | ASP | ASP | ASP | TYR |
Samples:
sample_1: Stress Response Peptide-2 2.2 mM
sample_conditions_1: pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ v3.2, Varian - collection
CCPNMR_Analysis v2.4, Vranken - chemical shift assignment, peak picking
SPARKY v3, Goddard - chemical shift assignment, peak picking, processing
CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution
NMR spectrometers:
- Varian Oxford 500 MHz