BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26864

Title: ScribPDZ4withp22phoxC10   PubMed: 27694890

Deposition date: 2016-07-30 Original release date: 2016-08-23

Authors: Umitsu, Masataka; Ikura, Mitsuhiko

Citation: Zheng, Weiyue; Umitsu, Masataka; Jagan, Ishaan; Tran, Charles; Ishiyama, Noboru; BeGora, Michael; Araki, Kiyomi; Ohashi, Pamela; Ikura, Mitsuhiko; Muthuswamy, Senthil. "An interaction between Scribble and the NADPH oxidase complex controls M1 macrophage polarization and function"  Nat. Cell. Biol. 18, 1244-1252 (2016).

Assembly members:
ScribPDZ4, polymer, 102 residues, Formula weight is not available
p22phox_peptide, polymer, 10 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ScribPDZ4: GSHMPGLRELCIQKAPGERL GISIRGGARGHAGNPRDPTD EGIFISKVSPTGAAGRDGRL RVGLRLLEVNQQSLLGLTHG EAVQLLRSVGDTLTVLVCDG FE
p22phox_peptide: NPIPVTDEVV

Data sets:
Data typeCount
15N chemical shifts82
1H chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ScribPDZ41
2p22peptideC102

Entities:

Entity 1, ScribPDZ4 102 residues - Formula weight is not available

1   GLYSERHISMETPROGLYLEUARGGLULEU
2   CYSILEGLNLYSALAPROGLYGLUARGLEU
3   GLYILESERILEARGGLYGLYALAARGGLY
4   HISALAGLYASNPROARGASPPROTHRASP
5   GLUGLYILEPHEILESERLYSVALSERPRO
6   THRGLYALAALAGLYARGASPGLYARGLEU
7   ARGVALGLYLEUARGLEULEUGLUVALASN
8   GLNGLNSERLEULEUGLYLEUTHRHISGLY
9   GLUALAVALGLNLEULEUARGSERVALGLY
10   ASPTHRLEUTHRVALLEUVALCYSASPGLY
11   PHEGLU

Entity 2, p22peptideC10 10 residues - Formula weight is not available

1   ASNPROILEPROVALTHRASPGLUVALVAL

Samples:

ScribPDZ4_15N: ScribPDZ4, [U-100% 15N], 0.2 mM; p22phox peptide 5.32 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCScribPDZ4_15Nisotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts