BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26866

Title: SNX27 PDZ domain   PubMed: 27595347

Deposition date: 2016-08-04 Original release date: 2016-08-30

Authors: Collins, Brett; Mas, Caroline; Clairfeuille, Thomas

Citation: Clairfeuille, Thomas; Mas, Caroline; Chan, Audrey; Yang, Zhe; Tello-Lafoz, Maria; Chandra, Mintu; Widagdo, Jocelyn; Kerr, Markus; Paul, Blessy; Merida, Isabel; Teasdale, Rohan; Pavlos, Nathan; Anggono, Victor; Collins, Brett. "A molecular code for endosomal recycling of phosphorylated cargos by the SNX27-retromer complex"  Nat. Struct. Mol. Biol. 23, 921-932 (2016).

Assembly members:
PDZ_domain, polymer, 101 residues, Formula weight is not available

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PDZ_domain: GSHGGSPRVVRIVKSESGYG FNVRGQVSEGGQLRSINGEL YAPLQHVSAVLPGGAADRAG VRKGDRILEVNGVNVEGATH KQVVDLIRAGEKELILTVLS V

Data sets:
Data typeCount
13C chemical shifts276
15N chemical shifts96
1H chemical shifts96

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ domain1

Entities:

Entity 1, PDZ domain 101 residues - Formula weight is not available

1   GLYSERHISGLYGLYSERPROARGVALVAL
2   ARGILEVALLYSSERGLUSERGLYTYRGLY
3   PHEASNVALARGGLYGLNVALSERGLUGLY
4   GLYGLNLEUARGSERILEASNGLYGLULEU
5   TYRALAPROLEUGLNHISVALSERALAVAL
6   LEUPROGLYGLYALAALAASPARGALAGLY
7   VALARGLYSGLYASPARGILELEUGLUVAL
8   ASNGLYVALASNVALGLUGLYALATHRHIS
9   LYSGLNVALVALASPLEUILEARGALAGLY
10   GLULYSGLULEUILELEUTHRVALLEUSER
11   VAL

Samples:

sample_1: PDZ domain, [U-100% 13C; U-100% 15N], 0.8 mM; Hepes 20 mM; NaCl 200 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker AMX 900 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts