BMRB Entry 26901
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26901
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Title: 1H, 13C and 15N backbone and side-chain resonance assignments of the ZnF UBP domain of USP20/VDU2 PubMed: 28091961
Deposition date: 2016-09-19 Original release date: 2017-02-15
Authors: Yang, Yuanyuan; Wen, Yi; Zhang, Naixia
Citation: Yang, Yuanyuan; Wen, Yi; Zhang, Naixia. "1H, 13C and 15N backbone and side-chain resonance assignments of the ZnF-UBP domain of USP20/VDU2" Biomol. NMR Assign. 11, 91-93 (2017).
Assembly members:
USP20, polymer, 102 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
USP20: GSHMGDSRDLCPHLDSIGEV
TKEDLLLKSKGTCQSCGVTG
PNLWACLQVACPYVGCGESF
ADHSTIHAQAKKHNLTVNLT
TFRLWCYACEKEVFLEQRLA
AP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 389 |
| 15N chemical shifts | 95 |
| 1H chemical shifts | 589 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | USP20 | 1 |
Entities:
Entity 1, USP20 102 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | GLY | ASP | SER | ARG | ASP | LEU | ||||
| 2 | CYS | PRO | HIS | LEU | ASP | SER | ILE | GLY | GLU | VAL | ||||
| 3 | THR | LYS | GLU | ASP | LEU | LEU | LEU | LYS | SER | LYS | ||||
| 4 | GLY | THR | CYS | GLN | SER | CYS | GLY | VAL | THR | GLY | ||||
| 5 | PRO | ASN | LEU | TRP | ALA | CYS | LEU | GLN | VAL | ALA | ||||
| 6 | CYS | PRO | TYR | VAL | GLY | CYS | GLY | GLU | SER | PHE | ||||
| 7 | ALA | ASP | HIS | SER | THR | ILE | HIS | ALA | GLN | ALA | ||||
| 8 | LYS | LYS | HIS | ASN | LEU | THR | VAL | ASN | LEU | THR | ||||
| 9 | THR | PHE | ARG | LEU | TRP | CYS | TYR | ALA | CYS | GLU | ||||
| 10 | LYS | GLU | VAL | PHE | LEU | GLU | GLN | ARG | LEU | ALA | ||||
| 11 | ALA | PRO |
Samples:
sample_1: D2O 10%; H2O 90%; USP20, [U-100% 13C; U-100% 15N], 0.6 – 0.8 mM; Na2HPO4/NaH2PO4 20 mM; NaCl 100 mM; DTT 2 mM; ZnSO4 50 uM
sample_2: D2O 100%; USP20, [U-100% 13C; U-100% 15N], 0.6 – 0.8 mM; Na2HPO4/NaH2PO4 20 mM; NaCl 100 mM; DTT 2 mM; ZnSO4 50 uM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298.2 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts