BMRB Entry 26905
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26905
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Title: backbone chemical shift assignment of S23C mutant of the Respiratory Syncytail Virus phosphoprotein PubMed: 28031463
Deposition date: 2016-09-22 Original release date: 2017-02-15
Authors: Cardone, Christophe; Lassoued, Safa; Galloux, Marie; Eleouet, Jean-Francois; Sizun, Christina
Citation: Pereira, Nelson; Cardone, Christophe; Lassoued, Safa; Galloux, Marie; Fix, Jenna; Assrir, Nadine; Lescop, Ewen; Bontems, Francois; Eleouet, Jean-Francois; Sizun, Christina. "New Insights into Structural Disorder in Human Respiratory Syncytial Virus Phosphoprotein and Implications for Binding of Protein Partners" J. Biol. Chem. 292, 2120-2131 (2017).
Assembly members:
P-S23C, polymer, 244 residues, 27413.0335 Da.
Natural source: Common Name: human RSV Taxonomy ID: 11250 Superkingdom: Viruses Kingdom: not available Genus/species: Orthopneumovirus HRSV
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
P-S23C: GSIMEKFAPEFHGEDANNRA
TKFLECIKGKFTSPKDPKKK
DSIISVNSIDIEVTKESPIT
SNSTIINPTNETDDNAGNKP
NYQRKPLVSFKEDPIPSDNP
FSKLYKETIETFDNNEEESS
YSYEEINDQTNDNITARLDR
IDEKLSEILGMLHTLVVASA
GPTSARDGIRDAMVGLREEM
IEKIRTEALMTNDRLEAMAR
LRNEESEKMAKDTSDEVSLN
PTSEKLNNLLEGNDSDNDLS
LEDF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 451 |
| 15N chemical shifts | 149 |
| 1H chemical shifts | 514 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | P-S23C, chain 1 | 1 |
| 2 | P-S23C, chain 2 | 1 |
| 3 | P-S23C, chain 3 | 1 |
| 4 | P-S23C, chain 4 | 1 |
Entities:
Entity 1, P-S23C, chain 1 244 residues - 27413.0335 Da.
hRSV phosphoprotein with S23C mutation. The three N-terminal residues (GSI) are left over from a cleaved GST-tag.
| 1 | GLY | SER | ILE | MET | GLU | LYS | PHE | ALA | PRO | GLU | ||||
| 2 | PHE | HIS | GLY | GLU | ASP | ALA | ASN | ASN | ARG | ALA | ||||
| 3 | THR | LYS | PHE | LEU | GLU | CYS | ILE | LYS | GLY | LYS | ||||
| 4 | PHE | THR | SER | PRO | LYS | ASP | PRO | LYS | LYS | LYS | ||||
| 5 | ASP | SER | ILE | ILE | SER | VAL | ASN | SER | ILE | ASP | ||||
| 6 | ILE | GLU | VAL | THR | LYS | GLU | SER | PRO | ILE | THR | ||||
| 7 | SER | ASN | SER | THR | ILE | ILE | ASN | PRO | THR | ASN | ||||
| 8 | GLU | THR | ASP | ASP | ASN | ALA | GLY | ASN | LYS | PRO | ||||
| 9 | ASN | TYR | GLN | ARG | LYS | PRO | LEU | VAL | SER | PHE | ||||
| 10 | LYS | GLU | ASP | PRO | ILE | PRO | SER | ASP | ASN | PRO | ||||
| 11 | PHE | SER | LYS | LEU | TYR | LYS | GLU | THR | ILE | GLU | ||||
| 12 | THR | PHE | ASP | ASN | ASN | GLU | GLU | GLU | SER | SER | ||||
| 13 | TYR | SER | TYR | GLU | GLU | ILE | ASN | ASP | GLN | THR | ||||
| 14 | ASN | ASP | ASN | ILE | THR | ALA | ARG | LEU | ASP | ARG | ||||
| 15 | ILE | ASP | GLU | LYS | LEU | SER | GLU | ILE | LEU | GLY | ||||
| 16 | MET | LEU | HIS | THR | LEU | VAL | VAL | ALA | SER | ALA | ||||
| 17 | GLY | PRO | THR | SER | ALA | ARG | ASP | GLY | ILE | ARG | ||||
| 18 | ASP | ALA | MET | VAL | GLY | LEU | ARG | GLU | GLU | MET | ||||
| 19 | ILE | GLU | LYS | ILE | ARG | THR | GLU | ALA | LEU | MET | ||||
| 20 | THR | ASN | ASP | ARG | LEU | GLU | ALA | MET | ALA | ARG | ||||
| 21 | LEU | ARG | ASN | GLU | GLU | SER | GLU | LYS | MET | ALA | ||||
| 22 | LYS | ASP | THR | SER | ASP | GLU | VAL | SER | LEU | ASN | ||||
| 23 | PRO | THR | SER | GLU | LYS | LEU | ASN | ASN | LEU | LEU | ||||
| 24 | GLU | GLY | ASN | ASP | SER | ASP | ASN | ASP | LEU | SER | ||||
| 25 | LEU | GLU | ASP | PHE |
Samples:
P-S23C-15N13C: P-S23C, [U-13C; U-15N; U-2H], 0.1 ± 1e-05 mM; sodium phosphate 20.0 ± 0.002 mM; sodium chloride 100.0 ± 0.01 mM
Condition: ionic strength: 0.100 M; pH: 6.500; pressure: 1.000 atm; temperature: 288.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | P-S23C-15N13C | isotropic | Condition |
| 3D HNCO | P-S23C-15N13C | isotropic | Condition |
| 3D HN(CO)CA | P-S23C-15N13C | isotropic | Condition |
| 3D HNCA | P-S23C-15N13C | isotropic | Condition |
| 3D HNCACB | P-S23C-15N13C | isotropic | Condition |
| 3D HN(CO)CACB | P-S23C-15N13C | isotropic | Condition |
| 3D HBHA(CO)NH | P-S23C-15N13C | isotropic | Condition |
Software:
CcpNmr_Analysis v2.2, CCPN - chemical shift assignment, data analysis, peak picking
Topspin v3.1, Bruker - collection, processing
NMR spectrometers:
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts