BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30013

Title: Solution structure of porcinelactoferricin in TFE

Deposition date: 2016-02-12 Original release date: 2017-02-13

Authors: Chan, M.; Tse, M.; Lo, K.; Sze, K.

Citation: Chan, M.; Tse, M.; Lo, K.; Sze, K.. "Solution structure of porcine lactoferricin in TFE"  . ., .-..

Assembly members:
Lactoferrin, polymer, 25 residues, 3040.595 Da.

Natural source:   Common Name: Pig   Taxonomy ID: 9823   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Lactoferrin: SKCRQWQSKIRRTNPIFCIR RASPT

Data sets:
Data typeCount
1H chemical shifts183

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 25 residues - 3040.595 Da.

1   SERLYSCYSARGGLNTRPGLNSERLYSILE
2   ARGARGTHRASNPROILEPHECYSILEARG
3   ARGALASERPROTHR

Samples:

sample_1: lactoferricin, 1H, 1 mM

sample_conditions_1: ionic strength: 0 mM; pH: 6.9; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, and Kollman - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

  • Bruker AVANCE 600 MHz spectromete 600 MHz