BMRB Entry 30023

Name: Solution structure of SdrG from Sphingomonas melonis Fr1
Published: 2016-07-13

Click here to enlarge.

PDB ID: 5ieb
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30023
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title: Solution structure of SdrG from Sphingomonas melonis Fr1 PubMed: 27396826

Deposition date: 2016-02-25 Original release date: 2016-07-13

Authors: Campagne, S.; Vorholt, J.; Allain, F.H.-T.

Citation: Campagne, Sebastien; Dintner, Sebastian; Gottschlich, Lisa; Thibault, Maxence; Bortfeld-Miller, Miriam; Kaczmarczyk, Andreas; Francez-Charlot, Anne; Allain, Frederic; Vorholt, Julia. "Role of the PFXFATG[G/Y] Motif in the Activation of SdrG, a Response Regulator Involved in the Alphaproteobacterial General Stress Response" Structure 24, 1237-1247 (2016).

Assembly members:
Sensory transduction regulatory protein, polymer, 130 residues, 13892.781 Da.

Natural source: Common Name: a-proteobacteria Taxonomy ID: 1090317 Superkingdom: Bacteria Kingdom: not available Genus/species: Sphingomonas Sphingomonas melonis

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Sensory transduction regulatory protein: MSALTQILIVEDEPLIAMML EDFLEVLDKTPVGTVDTVAG ALARVEDGGIDAAILDVNLR GGEKSTPVAEALAARDIPFV FATGGSDDSVDSRFRDRPVL QKPFTMDGVAKALAALLVPR GSVEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	502
15N chemical shifts	123
1H chemical shifts	860

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 130 residues - 13892.781 Da.

1	MET	SER	ALA	LEU	THR	GLN	ILE	LEU	ILE	VAL
2	GLU	ASP	GLU	PRO	LEU	ILE	ALA	MET	MET	LEU
3	GLU	ASP	PHE	LEU	GLU	VAL	LEU	ASP	LYS	THR
4	PRO	VAL	GLY	THR	VAL	ASP	THR	VAL	ALA	GLY
5	ALA	LEU	ALA	ARG	VAL	GLU	ASP	GLY	GLY	ILE
6	ASP	ALA	ALA	ILE	LEU	ASP	VAL	ASN	LEU	ARG
7	GLY	GLY	GLU	LYS	SER	THR	PRO	VAL	ALA	GLU
8	ALA	LEU	ALA	ALA	ARG	ASP	ILE	PRO	PHE	VAL
9	PHE	ALA	THR	GLY	GLY	SER	ASP	ASP	SER	VAL
10	ASP	SER	ARG	PHE	ARG	ASP	ARG	PRO	VAL	LEU
11	GLN	LYS	PRO	PHE	THR	MET	ASP	GLY	VAL	ALA
12	LYS	ALA	LEU	ALA	ALA	LEU	LEU	VAL	PRO	ARG
13	GLY	SER	VAL	GLU	HIS	HIS	HIS	HIS	HIS	HIS

Samples:

sample_1: NaCl 50 mM; NaPO4 10 mM; SdrG, [U-99% 13C; U-99% 15N], 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
1D H	sample_1	isotropic	sample_conditions_1

Software:

AMBER v12, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CARA, Keller and Wuthrich - data analysis

CYANA v3.96, P. Gunther - structure calculation

TOPSPIN v3.2, Bruker Biospin - collection

NMR spectrometers:

Bruker AvanceIII 500 MHz
Bruker AvanceII 900 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts

1	MET	SER	ALA	LEU	THR	GLN	ILE	LEU	ILE	VAL
2	GLU	ASP	GLU	PRO	LEU	ILE	ALA	MET	MET	LEU
3	GLU	ASP	PHE	LEU	GLU	VAL	LEU	ASP	LYS	THR
4	PRO	VAL	GLY	THR	VAL	ASP	THR	VAL	ALA	GLY
5	ALA	LEU	ALA	ARG	VAL	GLU	ASP	GLY	GLY	ILE
6	ASP	ALA	ALA	ILE	LEU	ASP	VAL	ASN	LEU	ARG
7	GLY	GLY	GLU	LYS	SER	THR	PRO	VAL	ALA	GLU
8	ALA	LEU	ALA	ALA	ARG	ASP	ILE	PRO	PHE	VAL
9	PHE	ALA	THR	GLY	GLY	SER	ASP	ASP	SER	VAL
10	ASP	SER	ARG	PHE	ARG	ASP	ARG	PRO	VAL	LEU
11	GLN	LYS	PRO	PHE	THR	MET	ASP	GLY	VAL	ALA
12	LYS	ALA	LEU	ALA	ALA	LEU	LEU	VAL	PRO	ARG
13	GLY	SER	VAL	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	SER	ALA	LEU	THR	GLN	ILE	LEU	ILE	VAL
2	GLU	ASP	GLU	PRO	LEU	ILE	ALA	MET	MET	LEU
3	GLU	ASP	PHE	LEU	GLU	VAL	LEU	ASP	LYS	THR
4	PRO	VAL	GLY	THR	VAL	ASP	THR	VAL	ALA	GLY
5	ALA	LEU	ALA	ARG	VAL	GLU	ASP	GLY	GLY	ILE
6	ASP	ALA	ALA	ILE	LEU	ASP	VAL	ASN	LEU	ARG
7	GLY	GLY	GLU	LYS	SER	THR	PRO	VAL	ALA	GLU
8	ALA	LEU	ALA	ALA	ARG	ASP	ILE	PRO	PHE	VAL
9	PHE	ALA	THR	GLY	GLY	SER	ASP	ASP	SER	VAL
10	ASP	SER	ARG	PHE	ARG	ASP	ARG	PRO	VAL	LEU
11	GLN	LYS	PRO	PHE	THR	MET	ASP	GLY	VAL	ALA
12	LYS	ALA	LEU	ALA	ALA	LEU	LEU	VAL	PRO	ARG
13	GLY	SER	VAL	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	SER	ALA	LEU	THR	GLN	ILE	LEU	ILE	VAL
2	GLU	ASP	GLU	PRO	LEU	ILE	ALA	MET	MET	LEU
3	GLU	ASP	PHE	LEU	GLU	VAL	LEU	ASP	LYS	THR
4	PRO	VAL	GLY	THR	VAL	ASP	THR	VAL	ALA	GLY
5	ALA	LEU	ALA	ARG	VAL	GLU	ASP	GLY	GLY	ILE
6	ASP	ALA	ALA	ILE	LEU	ASP	VAL	ASN	LEU	ARG
7	GLY	GLY	GLU	LYS	SER	THR	PRO	VAL	ALA	GLU
8	ALA	LEU	ALA	ALA	ARG	ASP	ILE	PRO	PHE	VAL
9	PHE	ALA	THR	GLY	GLY	SER	ASP	ASP	SER	VAL
10	ASP	SER	ARG	PHE	ARG	ASP	ARG	PRO	VAL	LEU
11	GLN	LYS	PRO	PHE	THR	MET	ASP	GLY	VAL	ALA
12	LYS	ALA	LEU	ALA	ALA	LEU	LEU	VAL	PRO	ARG
13	GLY	SER	VAL	GLU	HIS	HIS	HIS	HIS	HIS	HIS