BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30120

Title: Connexin 26 G12R mutant NMR structure   PubMed: 27378082

Deposition date: 2016-06-19 Original release date: 2016-09-23

Authors: Dowd, T.; Bargiello, T.

Citation: Batir, Y.; Bargiello, T.; Dowd, T.. "Structural studies of N-terminal mutants of Connexin 26 and Connexin 32 using (1)H NMR spectroscopy."  Arch. Biochem. Biophys. 608, 8-19 (2016).

Assembly members:
entity_1, polymer, 23 residues, 2472.863 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: XMDWGTLQTILGRVNKHSTS IGK

Data sets:
Data typeCount
13C chemical shifts41
1H chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 23 residues - 2472.863 Da.

1   ACEMETASPTRPGLYTHRLEUGLNTHRILE
2   LEUGLYARGVALASNLYSHISSERTHRSER
3   ILEGLYLYS

Samples:

sample_1: entity_1 1.0 ± 0.1 mM; KCL 100 ± 0.1 mM; TSP 100 ± 2 uM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 600 MHz