BMRB Entry 30123
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30123
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Title: Connexin 32 G12R N-Terminal Mutant PubMed: 27378082
Deposition date: 2016-06-21 Original release date: 2016-09-23
Authors: Dowd, T.; Barigello, T.
Citation: Batir, Y.; Bargiello, T.; Dowd, T.. "Structural studies of N-terminal mutants of Connexin 26 and Connexin 32 using (1)H NMR spectroscopy." Arch. Biochem. Biophys. 608, 8-19 (2016).
Assembly members:
entity_1, polymer, 23 residues, 2576.977 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: XMNWTGLYTLLSRVNRHSTA
IGR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 42 |
| 1H chemical shifts | 141 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 23 residues - 2576.977 Da.
| 1 | ACE | MET | ASN | TRP | THR | GLY | LEU | TYR | THR | LEU | ||||
| 2 | LEU | SER | ARG | VAL | ASN | ARG | HIS | SER | THR | ALA | ||||
| 3 | ILE | GLY | ARG |
Samples:
sample_1: entity_1 1.0 ± 0.1 mM; KCl 100 ± 0.1 mM; TSP 100 ± 2 uM
sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment
VNMR, Varian - collection
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 600 MHz