BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34015

Title: Myristoylated T41I/T78I mutant of M-PMV matrix protein   PubMed: 27725181

Deposition date: 2016-06-27 Original release date: 2016-07-21

Authors: Kroupa, T.; Hrabal, R.

Citation: Kroupa, Tomas; Langerova, Hana; Dolezal, Michal; Prchal, Jan; Spiwok, Vojtech; Hunter, Eric; Rumlova, Michaela; Hrabal, Richard; Ruml, Tomas. "Membrane interaction of the Mason-Pfizer monkey virus matrix protein and its budding deficient mutants"  J. Mol. Biol. 428, 4708-4722 (2016).

Assembly members:
entity_1, polymer, 124 residues, 14737.740 Da.
entity_MYR, non-polymer, 228.371 Da.

Natural source:   Common Name: MPMV   Taxonomy ID: 11855   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betaretrovirus MPMV

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):
entity_1: GQELSQHERYVEQLKQALKT RGVKVKYADLLKFFDFVKDI CPWFPQEGTIDIKRWRRVGD CFQDYYNTFGPEKVPVIAFS YWNLIKELIDKKEVNPQVMA AVAQTEEILKSNSQTDLEHH HHHH

Data sets:
Data typeCount
13C chemical shifts374
15N chemical shifts100
1H chemical shifts687

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_MYR2

Entities:

Entity 1, entity_1 124 residues - 14737.740 Da.

1   GLYGLNGLULEUSERGLNHISGLUARGTYR
2   VALGLUGLNLEULYSGLNALALEULYSTHR
3   ARGGLYVALLYSVALLYSTYRALAASPLEU
4   LEULYSPHEPHEASPPHEVALLYSASPILE
5   CYSPROTRPPHEPROGLNGLUGLYTHRILE
6   ASPILELYSARGTRPARGARGVALGLYASP
7   CYSPHEGLNASPTYRTYRASNTHRPHEGLY
8   PROGLULYSVALPROVALILEALAPHESER
9   TYRTRPASNLEUILELYSGLULEUILEASP
10   LYSLYSGLUVALASNPROGLNVALMETALA
11   ALAVALALAGLNTHRGLUGLUILELEULYS
12   SERASNSERGLNTHRASPLEUGLUHISHIS
13   HISHISHISHIS

Entity 2, entity_MYR - C14 H28 O2 - 228.371 Da.

1   MYR

Samples:

sample_1: DTT, none, 5 mM; myristoylated retroviral matrix protein, [U-13C; U-15N], 1 mM; phosphate, none, 100 mM; sodium choride, none, 300 mM

sample_2: DTT, none, 5 mM; Pf1 phage, none, 10 mg/mL; myristoylated retroviral matrix protein, [U-15N], 400 mM; phosphate, none, 50 mM; sodium choride, none, 150 mM

sample_conditions_1: ionic strength: 600 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQC IPAPsample_2anisotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

CcpNMR, CCPN - peak picking

TOPSPIN, Bruker Biospin - collection, processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation

NMR spectrometers:

  • Bruker AvanceIII 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts