BMRB Entry 4028
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4028
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Title: Chemical Shift Mapping of the RNA-binding Interface to the Multiple-RBD Protein Sex-Lethal: Chemical Shifts for the Protein-RNA Complex
Deposition date: 1997-03-15 Original release date: 2001-02-22
Authors: Lee, Andrew; Volkman, Brian; Robertson, Stephanie; Rudner, David; Barbash, Daniel; Cline, Thomas; Kanaar, Roland; Rio, Donald; Wemmer, David
Citation: Lee, Andrew; Volkman, Brian; Robertson, Stephanie; Rudner, David; Barbash, Daniel; Cline, Thomas; Kanaar, Roland; Rio, Donald; Wemmer, David. "Chemical Shift Mapping of the RNA-binding Interface to the Multiple-RBD Protein Sex-Lethal" Biochemistry 36, 14306-14317 (1997).
Assembly members:
Sex-lethal protein, polymer, 184 residues, Formula weight is not available
polypyrimidine tract 10-mer RNA, polymer, 10 residues, Formula weight is not available
Natural source: Common Name: fruitfly Taxonomy ID: 7215 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Sex-lethal protein: MGSDDLMNDPRASNTNLIVN
YLPQDMTDRELYALFRAIGP
INTCRIMRDYKTGYSFGYAF
VDFTSEMDSQRAIKVLNGIT
VRNKRLKVSYARPGGESIKD
TNLYVTNLPRTITDDQLDTI
FGKYGSIVQKNILRDKLTGR
PRGVAFVRYNKREEAQEAIS
ALNNVIPEGGSQPLSVRLAE
EHGK
polypyrimidine tract 10-mer RNA: GUUUUUUUUC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 308 |
| 15N chemical shifts | 149 |
| 1H chemical shifts | 261 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sex-lethal protein | 1 |
| 2 | RNA | 2 |
Entities:
Entity 1, Sex-lethal protein 184 residues - Formula weight is not available
| 1 | MET | GLY | SER | ASP | ASP | LEU | MET | ASN | ASP | PRO | ||||
| 2 | ARG | ALA | SER | ASN | THR | ASN | LEU | ILE | VAL | ASN | ||||
| 3 | TYR | LEU | PRO | GLN | ASP | MET | THR | ASP | ARG | GLU | ||||
| 4 | LEU | TYR | ALA | LEU | PHE | ARG | ALA | ILE | GLY | PRO | ||||
| 5 | ILE | ASN | THR | CYS | ARG | ILE | MET | ARG | ASP | TYR | ||||
| 6 | LYS | THR | GLY | TYR | SER | PHE | GLY | TYR | ALA | PHE | ||||
| 7 | VAL | ASP | PHE | THR | SER | GLU | MET | ASP | SER | GLN | ||||
| 8 | ARG | ALA | ILE | LYS | VAL | LEU | ASN | GLY | ILE | THR | ||||
| 9 | VAL | ARG | ASN | LYS | ARG | LEU | LYS | VAL | SER | TYR | ||||
| 10 | ALA | ARG | PRO | GLY | GLY | GLU | SER | ILE | LYS | ASP | ||||
| 11 | THR | ASN | LEU | TYR | VAL | THR | ASN | LEU | PRO | ARG | ||||
| 12 | THR | ILE | THR | ASP | ASP | GLN | LEU | ASP | THR | ILE | ||||
| 13 | PHE | GLY | LYS | TYR | GLY | SER | ILE | VAL | GLN | LYS | ||||
| 14 | ASN | ILE | LEU | ARG | ASP | LYS | LEU | THR | GLY | ARG | ||||
| 15 | PRO | ARG | GLY | VAL | ALA | PHE | VAL | ARG | TYR | ASN | ||||
| 16 | LYS | ARG | GLU | GLU | ALA | GLN | GLU | ALA | ILE | SER | ||||
| 17 | ALA | LEU | ASN | ASN | VAL | ILE | PRO | GLU | GLY | GLY | ||||
| 18 | SER | GLN | PRO | LEU | SER | VAL | ARG | LEU | ALA | GLU | ||||
| 19 | GLU | HIS | GLY | LYS |
Entity 2, RNA 10 residues - Formula weight is not available
| 1 | G | U | U | U | U | U | U | U | U | C |
Samples:
sample_one: Sex-lethal protein, [U-95% 13C; U-95% 15N], 0.5 – 1.0 mM; polypyrimidine tract 10-mer RNA0.5 – 1.0 mM; H2O 90%; D2O 10%; NaCl 30 mM; NaN3 0.02%; sodium phosphate buffer 30 mM
sample_condition_one: pH: 6.30 na; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
xwinnmr v1.1, Bruker - NMR data acquisition
FELIX v2.30, biosym - fourier transformation, peak picking, strip plotting, visualization
NMR spectrometers:
- Bruker AMX 600 MHz
Related Database Links:
| BMRB | 4029 |
| PDB | |
| GB | AAA28884 AAA28921 AAA28922 AAF46240 AAF46241 |
| PIR | B39725 |
| REF | NP_001027062 NP_001027063 NP_001027065 NP_001162686 NP_001162688 |
| SP | P19339 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts