BMRB Entry 4128
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4128
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: NMR Backbone Assignments of the Cyanobacterial Transcriptional Factor, SmtB, that Senses the Zinc Concentration in the Cell
Deposition date: 1998-03-23 Original release date: 1999-02-03
Authors: Morita, Hayato; Kosada, Takashi; Yamazaki, Toshio; Kyogoku, Yoshimasa; Hayashi, Hidenori
Citation: Morita, Hayato; Kosada, Takashi; Yamazaki, Toshio; Kyogoku, Yoshimasa; Hayashi, Hidenori. "NMR Backbone Assignments of the Cyanobacterial Transcriptional Factor, SmtB, that Senses the Zinc Concentration in the Cell" J. Biomol. NMR 12, 453-454 (1998).
Assembly members:
cyanobacterial metallothionein gene repressor, polymer, 121 residues, 13395.2 Da.
Natural source: Common Name: Synechococcus Taxonomy ID: 1140 Superkingdom: Eubacteria Kingdom: not available Genus/species: Synechococcus PCC7942
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
cyanobacterial metallothionein gene repressor: TKPVLQDGETVVCQGTHAAI
ASELQAIAPEVAQSLAEFFA
VLADPNRLRLLSLLARSELC
VGDLAQAIGVSESAVSHQLR
SLRNLRLVSYRKQGRHVYYQ
LQDHHIVALYQNALDHLQEC
R
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 119 |
| 15N chemical shifts | 116 |
| 1H chemical shifts | 116 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SmtB subunit one | 1 |
| 2 | SmtB subunit two | 1 |
Entities:
Entity 1, SmtB subunit one 121 residues - 13395.2 Da.
| 1 | THR | LYS | PRO | VAL | LEU | GLN | ASP | GLY | GLU | THR | ||||
| 2 | VAL | VAL | CYS | GLN | GLY | THR | HIS | ALA | ALA | ILE | ||||
| 3 | ALA | SER | GLU | LEU | GLN | ALA | ILE | ALA | PRO | GLU | ||||
| 4 | VAL | ALA | GLN | SER | LEU | ALA | GLU | PHE | PHE | ALA | ||||
| 5 | VAL | LEU | ALA | ASP | PRO | ASN | ARG | LEU | ARG | LEU | ||||
| 6 | LEU | SER | LEU | LEU | ALA | ARG | SER | GLU | LEU | CYS | ||||
| 7 | VAL | GLY | ASP | LEU | ALA | GLN | ALA | ILE | GLY | VAL | ||||
| 8 | SER | GLU | SER | ALA | VAL | SER | HIS | GLN | LEU | ARG | ||||
| 9 | SER | LEU | ARG | ASN | LEU | ARG | LEU | VAL | SER | TYR | ||||
| 10 | ARG | LYS | GLN | GLY | ARG | HIS | VAL | TYR | TYR | GLN | ||||
| 11 | LEU | GLN | ASP | HIS | HIS | ILE | VAL | ALA | LEU | TYR | ||||
| 12 | GLN | ASN | ALA | LEU | ASP | HIS | LEU | GLN | GLU | CYS | ||||
| 13 | ARG |
Samples:
sample_one: cyanobacterial metallothionein gene repressor, [U-15N];[U-13C,U-15N];[15N]-His;[15N]-Lys;[15N]-Arg;[15N]-Val;[15N]-Leu;[15N]-Cys, 0.6 mM; H2O 90%; D2O 10%
sample_conditions_one: pH: 6.0; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D_1H-15N_HSQC | sample_one | not available | sample_conditions_one |
| Three-dimensional experiments | sample_one | not available | sample_conditions_one |
| 3D_HNCACB | sample_one | not available | sample_conditions_one |
| 3D_CBCA(CO)NH | sample_one | not available | sample_conditions_one |
| 3D_HNCA | sample_one | not available | sample_conditions_one |
| 3D_HN(CO)CA | sample_one | not available | sample_conditions_one |
Software:
NMRPipe v1.6, NIH - peak picking and assignments
NMR spectrometers:
- Bruker DMX 500 MHz
Related Database Links:
| BMRB | 4306 |
| PDB | |
| DBJ | BAD78452 |
| EMBL | CAA45872 |
| GB | AAT01609 AAT01610 AAT01611 AAT01612 AAT01613 |
| REF | WP_011242576 |
| SP | P30340 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts