BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4465

Title: The Structural role of the Copper-coordinating and Surface-exposed Histidine Residue in the Blue Copper Protein Azurin   PubMed: 10835281

Deposition date: 1999-11-24 Original release date: 2000-06-19

Authors: Jeuken, Lars; Ubbink, Marcellus; Bitter, Johannes; van Vliet, Pieter; Meyer-Klaucke, W.; Canters, Gerard

Citation: Jeuken, Lars; Ubbink, Marcellus; Bitter, Johannes; van Vliet, Pieter; Meyer-Klaucke, W.; Canters, Gerard. "The Structural role of the Copper-coordinating and Surface-exposed Histidine Residue in the Blue Copper Protein Azurin"  J. Mol. Biol. 299, 737-755 (2000).

Assembly members:
P. Aeruginosa HIs117Gly azurin, polymer, 128 residues, Formula weight is not available
CU1, non-polymer, 63.546 Da.

Natural source:   Common Name: fluorescent pseudomonads   Taxonomy ID: 287   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
P. Aeruginosa HIs117Gly azurin: AECSVDIQGNDQMQFNTNAI TVDKSCKQFTVNLSHPGNLP KNVMGHNWVLSTAADMQGVV TDGMASGLDKDYLKPDDSRV IAHTKLIGSGEKDSVTFDVS KLKEGEQYMFFCTFPGGSAL MKGTLTLK

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts245
15N chemical shifts123

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1His117Gly azurin1
2CU2

Entities:

Entity 1, His117Gly azurin 128 residues - Formula weight is not available

1   ALAGLUCYSSERVALASPILEGLNGLYASN
2   ASPGLNMETGLNPHEASNTHRASNALAILE
3   THRVALASPLYSSERCYSLYSGLNPHETHR
4   VALASNLEUSERHISPROGLYASNLEUPRO
5   LYSASNVALMETGLYHISASNTRPVALLEU
6   SERTHRALAALAASPMETGLNGLYVALVAL
7   THRASPGLYMETALASERGLYLEUASPLYS
8   ASPTYRLEULYSPROASPASPSERARGVAL
9   ILEALAHISTHRLYSLEUILEGLYSERGLY
10   GLULYSASPSERVALTHRPHEASPVALSER
11   LYSLEULYSGLUGLYGLUGLNTYRMETPHE
12   PHECYSTHRPHEPROGLYGLYSERALALEU
13   METLYSGLYTHRLEUTHRLEULYS

Entity 2, CU - Cu - 63.546 Da.

1   CU1

Samples:

Typical_Sample: P. Aeruginosa HIs117Gly azurin, [U-15N], 3 – 5 mM

Typical_Sample_Conditions: pH: 5.5; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCTypical_Samplenot availableTypical_Sample_Conditions
1H-15N TROSYTypical_Samplenot availableTypical_Sample_Conditions
1H-15N TOCSYTypical_Samplenot availableTypical_Sample_Conditions
1H-1H TOCSYTypical_Samplenot availableTypical_Sample_Conditions
1H-15N NOESYTypical_Samplenot availableTypical_Sample_Conditions
1H-1H NOESYTypical_Samplenot availableTypical_Sample_Conditions

Software:

azara - FID processing

ANSIG v3.3 - peak assignments and peak integration

NMR spectrometers:

  • Bruker Avance DMX 600 MHz

Related Database Links:

BMRB 1210 1211 1212 1213 1214 1215 1216 1217 1218 1219 18254
PDB
DBJ BAK87490 BAP24727 BAP53499 BAQ42761 BAR70381
EMBL CAA30279 CAW30062 CCQ86145 CDH73668 CDH79986
GB AAA25730 AAG08307 AAP03090 AAT49489 ABJ14307
PRF 671048A
REF NP_253609 WP_003095591 WP_003141697 WP_031690945 WP_033949908
SP B3EWN9 P00282

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts